NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1436435 Query DataSets for GSM1436435
Status Public on Jul 16, 2014
Title ST-01012991_S-001146734_8450989072_C
Sample type RNA
 
Source name Mouse lung tissue
Organism Mus musculus
Characteristics treatment: LPS
time collected: 20
Extracted molecule total RNA
Extraction protocol For mRNA isolation lung tissue was immersed in RNAlater (Qiagen) and total RNA was then extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA quality (RIN>=7) was confirmed using an Agilent 2100 Bioanalyzer.
Label Cy3
Label protocol RNA from all biological samples was labeled at once using the Ambion TotalPrep-96 RNA Amplification Kit according to the manufacturer's instructions.
 
Hybridization protocol The samples were then blinded, randomized to chip position and hybridized to the Illumina MouseRef-8 v2.0 Expression BeadChips. All chips had one array dedicated as an RNA control.
Scan protocol The chips were read using standard illumina protocols using an Illumina iScan reader.
Data processing VSN normalized, background corrected
 
Submission date Jul 14, 2014
Last update date Jul 16, 2014
Contact name Jen-hwa Chu
E-mail(s) jen-hwa.chu@yale.edu
Organization name Yale University School of Medicine
Department Pulmonary, Critical Care and Sleep Medicine
Street address PO Box 208057
City New Haven
State/province CT
ZIP/Postal code 06520
Country USA
 
Platform ID GPL6885
Series (2)
GSE59404 CIRCADIAN RHYTHM REPROGRAMMING DURING LUNG INFLAMMATION/ Microarray Experiment #2
GSE59444 CIRCADIAN RHYTHM REPROGRAMMING DURING LUNG INFLAMMATION

Data table header descriptions
ID_REF
VALUE normalized signal intensity
RAW_VALUE raw signal intensity
BEAD_STDERR the standard error of the probe measurements
Detection_Pval the detection p-value of the probe
Avg_NBEADS Number of beads for the probe

Data table
ID_REF VALUE RAW_VALUE BEAD_STDERR Detection_Pval Avg_NBEADS
ILMN_2896528 11.0868078582425 1642.1 312.203531568751 0 41
ILMN_2721178 9.067418235954 518.5 133.788419229767 0 45
ILMN_3033922 10.4780956807467 1128.7 241.175577700148 0 39
ILMN_3092673 12.7088236501364 4741.5 904.60411245583 0 37
ILMN_2816356 2.43802436023792 122.1 46.8859335835387 0.58396 37
ILMN_2808939 9.26021208015575 571.1 164.222929166423 0 34
ILMN_2634564 9.42903251213932 623.3 164.105 0 25
ILMN_2737647 2.33044999870862 120.7 35.5307015410616 0.63409 32
ILMN_2734484 9.21384344309615 557.8 154.768193243961 0 40
ILMN_2952292 7.3031159479012 258.9 82.9730124799624 0 38
ILMN_2699078 2.71371274139296 125.9 37.4298590967158 0.46742 43
ILMN_1213681 8.27487940906074 363 89.1721548747141 0 45
ILMN_2735413 4.34247339185239 153.9 48.4953552415074 0.0401 30
ILMN_2735415 6.47827011897943 211.7 81.3859957302238 0 29
ILMN_2891688 10.4515987757612 1110.9 230.684309544451 0 30
ILMN_2637698 11.052351367068 1606.9 233.947913929575 0 28
ILMN_2674228 8.57435849615138 412 132.154870602638 0 54
ILMN_2601546 3.08595526437702 131.5 52.370553214569 0.34586 39
ILMN_1230831 2.00065547256323 116.7 40.2156825753337 0.73183 45
ILMN_2848071 3.50473896595671 138.4 56.4571493701196 0.21053 35

Total number of rows: 25697

Table truncated, full table size 1516 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap