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Sample GSM1436437 Query DataSets for GSM1436437
Status Public on Jul 16, 2014
Title ST-01013002_S-001146764_8450989072_E
Sample type RNA
 
Source name Mouse lung tissue
Organism Mus musculus
Characteristics treatment: untreated
time collected: 8
Extracted molecule total RNA
Extraction protocol For mRNA isolation lung tissue was immersed in RNAlater (Qiagen) and total RNA was then extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA quality (RIN>=7) was confirmed using an Agilent 2100 Bioanalyzer.
Label Cy3
Label protocol RNA from all biological samples was labeled at once using the Ambion TotalPrep-96 RNA Amplification Kit according to the manufacturer's instructions.
 
Hybridization protocol The samples were then blinded, randomized to chip position and hybridized to the Illumina MouseRef-8 v2.0 Expression BeadChips. All chips had one array dedicated as an RNA control.
Scan protocol The chips were read using standard illumina protocols using an Illumina iScan reader.
Data processing VSN normalized, background corrected
 
Submission date Jul 14, 2014
Last update date Jul 16, 2014
Contact name Jen-hwa Chu
E-mail(s) jen-hwa.chu@yale.edu
Organization name Yale University School of Medicine
Department Pulmonary, Critical Care and Sleep Medicine
Street address PO Box 208057
City New Haven
State/province CT
ZIP/Postal code 06520
Country USA
 
Platform ID GPL6885
Series (2)
GSE59404 CIRCADIAN RHYTHM REPROGRAMMING DURING LUNG INFLAMMATION/ Microarray Experiment #2
GSE59444 CIRCADIAN RHYTHM REPROGRAMMING DURING LUNG INFLAMMATION

Data table header descriptions
ID_REF
VALUE normalized signal intensity
RAW_VALUE raw signal intensity
BEAD_STDERR the standard error of the probe measurements
Detection_Pval the detection p-value of the probe
Avg_NBEADS Number of beads for the probe

Data table
ID_REF VALUE RAW_VALUE BEAD_STDERR Detection_Pval Avg_NBEADS
ILMN_2896528 12.1657202054287 4645.8 799.869703369242 0 38
ILMN_2721178 9.92872749578949 1094.5 242.127739914286 0 41
ILMN_3033922 10.6690036314571 1735.6 352.442326409584 0 45
ILMN_3092673 12.8505825981443 7384.3 1463.00016929972 0 51
ILMN_2816356 4.38145915239612 156.3 46.9677573980278 0.03634 35
ILMN_2808939 9.27331354850569 745.4 125.24343485788 0 39
ILMN_2634564 8.37040315581893 463 153.521155643123 0 30
ILMN_2737647 1.97986057602809 120.3 37.4430593301349 0.67293 33
ILMN_2734484 9.20094548050091 715.7 222.246039157057 0 41
ILMN_2952292 6.35247361545153 218.5 77.6644606367674 0.00125 46
ILMN_2699078 2.97762616797594 133.5 58.3546454277635 0.30702 43
ILMN_1213681 9.26353671577713 741.3 167.662673914023 0 54
ILMN_2735413 3.4717685953676 140.9 45.5888491629258 0.15915 33
ILMN_2735415 3.97098857934839 149 56.700788565945 0.06767 24
ILMN_2891688 9.72434310949164 968.2 248.029132200232 0 45
ILMN_2637698 10.7232487319543 1796.8 353.239065789728 0 34
ILMN_2674228 8.68103580335318 541 146.956623549264 0 45
ILMN_2601546 2.34140868231554 124.8 40.6197535073762 0.53133 47
ILMN_1230831 2.72993577759865 130 50.012473524112 0.38471 37
ILMN_2848071 4.11031108991093 151.4 57.9047493734322 0.05639 31

Total number of rows: 25697

Table truncated, full table size 1517 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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