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Sample GSM1441282 Query DataSets for GSM1441282
Status Public on Aug 08, 2014
Title H3K27Ac_CD8.ucsc
Sample type SRA
 
Source name T CD8+ cells
Organism Mus musculus
Characteristics cell type: T CD8+ cells
passages: In vivo FACS purified cells
strain: C57BL/6
chip antibody: H3K27Ac
Growth protocol Cells were fixated and FACS sorted according to stablished markers
Extracted molecule genomic DNA
Extraction protocol Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.
A new protocol (iChIP) was designed to perform iCHIP from small cell numbers
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 1500
 
Data processing Basecalls performed using CASAVA version 1.4
Genome_build: mm9
Supplementary_files_format_and_content: wig files were generated using …; Scores represent …
 
Submission date Jul 21, 2014
Last update date May 15, 2019
Contact name Ido Amit
E-mail(s) ido.amit@weizmann.ac.il
Phone 972-8-9343338
Organization name Weizmann Institute of Science
Department Immunology
Street address 234 Herzl st.
City Rehovot
ZIP/Postal code 760001
Country Israel
 
Platform ID GPL18480
Series (2)
GSE59636 Chromatin state dynamics during blood formation (ChIP-Seq)
GSE60103 Chromatin state dynamics during blood formation
Relations
BioSample SAMN02929031
SRA SRX658387

Supplementary file Size Download File type/resource
GSM1441282_K27Ac_CD8.ucsc.bigWig 44.4 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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