|
Status |
Public on Jul 23, 2014 |
Title |
MiR-21 knockout replicate 1 |
Sample type |
RNA |
|
|
Source name |
MiR-21-KO
|
Organism |
Mus musculus |
Characteristics |
tissue: colon mucosa strain: c57BL/6 genotype: MiR-21 knockout group: MiR-21-KO
|
Treatment protocol |
MiR-21-/- (21KO) mice on C57BL/6 background were kindly provided by Dr. Eric Olson (Patrick, et al. J Clin Invest. 2010;120:3912-3916.). The miR-21-/- mice do not develop spontaneous colitis. Gene expression profiles were established for normal miR-21-/- mice and wild type c57BL/6 mice (WT) from Taconic Farm Inc. (Hudson, NY)
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
Cy3
|
Label protocol |
not provided
|
|
|
Hybridization protocol |
Hybridized to Illumina MouseRef-8 v2.0 expression beadchip.
|
Scan protocol |
not provided
|
Description |
JK5
|
Data processing |
raw data were processed using the R/Bioconductor package limma for background correction and quantile normalization
|
|
|
Submission date |
Jul 22, 2014 |
Last update date |
Jul 23, 2014 |
Contact name |
Yong Huang |
E-mail(s) |
yh9fj@virginia.edu
|
Phone |
(434) 243-0842
|
Organization name |
University of Viginia
|
Department |
Medicine
|
Street address |
1340 Jefferson Park Ave
|
City |
Charlottesville |
State/province |
VA |
ZIP/Postal code |
22908 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (2) |
GSE59648 |
Divergent influence of microRNA-21 deletion on murine colitis phenotypes [mRNA] |
GSE59651 |
Divergent influence of microRNA-21 deletion on murine colitis phenotypes |
|