|
| Status |
Public on Dec 01, 2006 |
| Title |
DEC205_Flt3L_3 |
| Sample type |
RNA |
| |
|
| Source name |
DEC205_Flt3L_3
|
| Organism |
Mus musculus |
| Characteristics |
Spleen Dendritic Cells from Flt3L Melanom injected mice (12days), negatively enriched with CD19, CD90.2 and DX5 beads (Milteny) and sorted for DEC205/CD205 positive DCs (33D1A647, DEC205bio/SAPECy7/CD11cPE (High)), (equivalent to CD11c(High),CD8 Positive spleen DCs
C57BL/6 mice, Gender Female, Age 6-8 weeks
|
| Treatment protocol |
Flt3L Melanom injected, sacrified day 10-12
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Mini prep extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip MOE-430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner
|
| Description |
Gene expression data from purified splenic DCs from 20 Flt3L Melanom injected C57BL6 mice
|
| Data processing |
The data were analyzed with GCOS (same algorithm as MAS5.0 and newer version) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Nov 09, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Diana Dudziak |
| Organization name |
The Rockefeller University
|
| Lab |
Laboratory of Molecular Immunology
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE6259 |
Differential antigen processing by dendritic cell subsets in vivo |
|
| Relations |
| Reanalyzed by |
GSE37995 |
| Reanalyzed by |
GSE45704 |
| Reanalyzed by |
GSE119085 |
