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Sample GSM1442337 Query DataSets for GSM1442337
Status Public on Nov 11, 2014
Title KO OC2 Adult 3
Sample type RNA
Source name OC2-KO adult retina
Organism Mus musculus
Characteristics tissue: retina
Stage: adult
onecut genotype: OC2-KO
Treatment protocol Retinas were isolated from adult eyes and rinsed in 1X PBS.
Growth protocol Littermates were allowed to develop normally in utero until maturity
Extracted molecule total RNA
Extraction protocol Trireagent extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Total RNA wa amplified, biotinylated, and fragmented using the Ambion MessageAmp III RNA amplification kit according to manufacturer's instructions.
Hybridization protocol Amplified RNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays at the Iowa State University GeneChip Facility.
Scan protocol Amplified RNA was scanned from Affymetrix GeneChip Mouse Genome 430 2.0 arrays at the Iowa State University GeneChip Facility.
Description Gene expression information from isolated adult retinas
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Submission date Jul 22, 2014
Last update date Nov 12, 2014
Contact name Jeffrey M Trimarchi
Phone 2172190210
Organization name Iowa State University
Department GDCB
Lab Trimarchi
Street address 3188 Molecular Biology Building
City Ames
State/province IA
ZIP/Postal code 50014
Country USA
Platform ID GPL1261
Series (1)
GSE57918 Expression data from adult Onecut2 WT and KO animals

Data table header descriptions
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)

Data table
AFFX-BioB-5_at 1938.08 P 4.42246e-05
AFFX-BioB-M_at 3303 P 4.42246e-05
AFFX-BioB-3_at 2283.32 P 4.42873e-05
AFFX-BioC-5_at 6156.45 P 5.16732e-05
AFFX-BioC-3_at 8997.15 P 8.14279e-05
AFFX-BioDn-5_at 14113 P 4.42873e-05
AFFX-BioDn-3_at 19442.5 P 4.42873e-05
AFFX-CreX-5_at 88229.8 P 5.16732e-05
AFFX-CreX-3_at 88161.2 P 4.42873e-05
AFFX-DapX-5_at 4.418 A 0.994446
AFFX-DapX-M_at 1.36327 A 0.995543
AFFX-DapX-3_at 53.5566 A 0.686367
AFFX-LysX-5_at 31.2546 A 0.834244
AFFX-LysX-M_at 4.1957 A 0.932492
AFFX-LysX-3_at 12.8064 A 0.891122
AFFX-PheX-5_at 3.63172 A 0.971597
AFFX-PheX-M_at 6.07046 A 0.932407
AFFX-PheX-3_at 46.3572 A 0.783576
AFFX-ThrX-5_at 41.6741 A 0.783596
AFFX-ThrX-M_at 25.8722 A 0.7842

Total number of rows: 45101

Table truncated, full table size 1373 Kbytes.

Supplementary file Size Download File type/resource
GSM1442337_KO_OC2_Adult_3.CEL.gz 2.5 Mb (ftp)(http) CEL
GSM1442337_KO_OC2_Adult_3.CHP.gz 427.9 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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