|
| Status |
Public on Nov 18, 2006 |
| Title |
Breast Cancer Post-Treatment 2 |
| Sample type |
RNA |
| |
|
| Source name |
Tumor mammary tissue 2 after treatment
|
| Organism |
Homo sapiens |
| Characteristics |
Core-needle biopsy was taken from primary breast tumors after 7 days of treatment with hydralazine and magnesium valproate prior to the first course of doxorubicin plus cyclophosphamide.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard CodeLink protocol from 2 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 37C on Human Whole Genome CodeLink Bioarray. Stain and post-hybridization washes were performed according to the manufacturer's instructions.
|
| Scan protocol |
Bioarrays were scanned with a GenePix Personal 4100A scanner.
|
| Description |
Gene expression data from a primary breast tumor after 7 days of treatment with hydralazine and magnesium valproate prior to the first course of doxorubicin plus cyclophosphamide.
|
| Data processing |
Expression values were globally normalized to the median expression value of the whole array spots. To circumvent division by zero, values below three times background value were taken as 5.
|
| |
|
| Submission date |
Nov 16, 2006 |
| Last update date |
Nov 17, 2006 |
| Contact name |
Carlos Pérez-Plasencia |
| E-mail(s) |
car_plas@yahoo.com
|
| Phone |
+(52) 55 56280400
|
| Fax |
+(52) 55 56280486
|
| URL |
http://www.incan.edu.mx/
|
| Organization name |
INSTITUTO NACIONAL DE CANCEROLOGIA
|
| Department |
BASIC RESEARCH
|
| Lab |
EPIGENETICS
|
| Street address |
Av. San Fernando 22
|
| City |
México |
| State/province |
D.F. |
| ZIP/Postal code |
14080 |
| Country |
Mexico |
| |
|
| Platform ID |
GPL2895 |
| Series (1) |
| GSE6304 |
Gene Expression Profiles of Epigenetic treatment in primary breast cancer. |
|
