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Sample GSM1449054 Query DataSets for GSM1449054
Status Public on Jul 30, 2014
Title WT proliferating SVZ cells-#1
Sample type RNA
 
Source name Subventricular zone cells
Organism Mus musculus
Characteristics growth media: Self-renewal media
age: post natal day 9
genotype/variation: Jmjd3F/+
Growth protocol SVZ NSCs were passaged 5 times in proliferation medium(DMEM/F12/N2 – Invitrogen, 5% FCS - Hyclone, 20 ng ml-1 EGF, 20 ng ml-1 bFGF, 35 ?g ml-1 bovine pituitary extract – Peprotech) before switching to differentiation media (same as proliferation media exc
Extracted molecule total RNA
Extraction protocol Total RNA was isolated by Trizol (Life Technologies), purified using the RNeasy Plus Mini Kit (Qiagen) and quantified using the NanoDrop spectrophotometer. Samples were checked for quality using an Agilent TapeStation
Label biotin
Label protocol Biotinylated cRNA was generated from total RNA using an Ambion Illumina RNA amplification kit (Ambion)
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Data processing The data were normalised using quantile normalisation with IlluminaGUI in R
 
Submission date Jul 29, 2014
Last update date Jul 30, 2014
Contact name John Liu
E-mail(s) john.liu@ucsf.edu
Organization name UCSF
Street address 35 Medical Center Way
City SAN FRANCISCO
State/province California
ZIP/Postal code 94143
Country USA
 
Platform ID GPL6885
Series (1)
GSE59888 Epigenetic activation of neuronal gene expression by JMJD3 is required for postnatal and adult brain neurogenesis

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_2607609 2286.666667
ILMN_1238674 142.4166667
ILMN_3062534 153.5
ILMN_3140158 1575
ILMN_2674533 359.5
ILMN_2680307 346.5
ILMN_2935307 142.4166667
ILMN_2910106 613
ILMN_1215893 690.3333333
ILMN_2732092 301.5
ILMN_2603725 4096.666667
ILMN_2664884 376.6666667
ILMN_3003575 167
ILMN_1229397 161.6666667
ILMN_1225873 244.3333333
ILMN_2899591 1126.416667
ILMN_2658355 156.6666667
ILMN_2682763 161.6666667
ILMN_2521511 155.6666667
ILMN_2691286 159.5

Total number of rows: 25697

Table truncated, full table size 566 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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