|
Status |
Public on Sep 09, 2015 |
Title |
MTS 1 (KR-N+/-) |
Sample type |
RNA |
|
|
Source name |
KR-Notch1+/-
|
Organism |
Mus musculus |
Characteristics |
tissue: Oral tumor tissue
|
Treatment protocol |
At the time of isolation, tumors were placed in Trizol, snap frozen and stored at -80 C.
|
Growth protocol |
Oral tumors were allowed to grow for 21d and were isolated.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treated. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
Biological Replicate 1
|
Data processing |
The data were normalised using quantile normalisation with background subtrations in Genome Studio. Data was exported and analyzed by Partek Genomics Suite 6.
|
|
|
Submission date |
Jul 31, 2014 |
Last update date |
Sep 09, 2015 |
Contact name |
Michael Spiotto |
E-mail(s) |
mspiotto@radonc.uchicago.edu
|
Organization name |
The University of Chicago
|
Department |
Radiation Oncology
|
Lab |
KCBD 6142
|
Street address |
900 E. 57th St
|
City |
Chicago |
State/province |
IL |
ZIP/Postal code |
60637 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE59951 |
Expression analysis of KHR and KR oral tumors with intact or deficient Notch1 |
|