|
| Status |
Public on Sep 09, 2015 |
| Title |
MTS 20 (KHR-N-/-) |
| Sample type |
RNA |
| |
|
| Source name |
KHR-Notch1 -/-
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Oral tumor tissue
|
| Treatment protocol |
At the time of isolation, tumors were placed in Trizol, snap frozen and stored at -80 C.
|
| Growth protocol |
Oral tumors were allowed to grow for 21d and were isolated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treated. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Biological Replicate 2
|
| Data processing |
The data were normalised using quantile normalisation with background subtrations in Genome Studio. Data was exported and analyzed by Partek Genomics Suite 6.
|
| |
|
| Submission date |
Jul 31, 2014 |
| Last update date |
Sep 09, 2015 |
| Contact name |
Michael Spiotto |
| E-mail(s) |
mspiotto@radonc.uchicago.edu
|
| Organization name |
The University of Chicago
|
| Department |
Radiation Oncology
|
| Lab |
KCBD 6142
|
| Street address |
900 E. 57th St
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE59951 |
Expression analysis of KHR and KR oral tumors with intact or deficient Notch1 |
|
