|
| Status |
Public on Nov 12, 2007 |
| Title |
Muscle non-HRT baseline 1302 |
| Sample type |
RNA |
| |
|
| Source name |
vastus lateralis muscle, non-HRT user, baseline
|
| Organism |
Homo sapiens |
| Characteristics |
Gender: female
Age: 57 at onset of intervention
menopausal status: early postmenopausal
|
| Treatment protocol |
needle muscle biopsy, immediately frozen in liquid nitrogen after extrication from biobsy needle, stored at - 70 degC until RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Frozen muscle biopsy samples were homogenized in Trizol-reagent (Invitrogen Inc.) with aid of FastPrep FP120 -apparatus (MP Biomedicals). Manufacturer’s protocol was followed through the isolation procedure.
|
| Label |
Cy3
|
| Label protocol |
Illumina RNA amplification kit (Ambion Inc., #I1755) was used according to manufacturer’s instructions. Input amount: 500 ng of total RNA.
|
| |
|
| Hybridization protocol |
Employing protocols by Illumina Inc.; hybridization: 17 h at 55 degC
|
| Scan protocol |
Using an Illumina BeadReader containin two lasers: 532 nm and 635 nm and performing with 0.8 microns resolution. The data was acquired by the BeadStudio Direct Hybridization V.1.5.0.34
|
| Description |
This sample presents baseline sample of ID_1302 individual, who belonged to non-hormone replacement therapy group (non-HRT) of "HRT and excercise"-intervention
|
| Data processing |
The raw data was acquired by the BeadStudio Direct Hybridization V.1.5.0.34 (Illumina inc) and quantile normalized with Inforsense KDE 3.1 (InforSense Inc.) software
|
| |
|
| Submission date |
Nov 22, 2006 |
| Last update date |
Nov 12, 2007 |
| Contact name |
Eija Pöllänen |
| E-mail(s) |
eija.pollanen@jyu.fi
|
| Phone |
+358-14-2604531
|
| Fax |
+358-14-2604600
|
| Organization name |
University of Jyväskylä
|
| Department |
Department of Health Sciences
|
| Street address |
PO Box 35/Viveca
|
| City |
Jyväskylä |
| ZIP/Postal code |
40014 |
| Country |
Finland |
| |
|
| Platform ID |
GPL2700 |
| Series (1) |
| GSE6348 |
Expression data of skeletal muscle from HRT users and non-users |
|
