|
| Status |
Public on Oct 15, 2014 |
| Title |
SK_CD14_DC_X221 |
| Sample type |
RNA |
| |
|
| Source name |
SK_CD14_DC
|
| Organism |
Homo sapiens |
| Characteristics |
donor id: X221
tissue: Skin
cell subtype: CD14+ dendritic cells (DCs)
|
| Treatment protocol |
None
|
| Growth protocol |
Monocytes and dendritic cell subsets were purified by FACS sorting from primary samples for transcription analysis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen RNeasy Micro RNA kit. Amplification was performed using Nugen Pico Ovation kit.
|
| Label |
biotin
|
| Label protocol |
Standard labelling protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
X221.14
|
| Data processing |
Expression data was exported from Illlumina GenomeStudio software without background subtraction and was then quantile normalized and log (base 2) transformed in R (v. 2.15.2) using Bioconductor (v.2.18.0) lumi (v.2.10.0) package.
|
| |
|
| Submission date |
Aug 11, 2014 |
| Last update date |
Oct 23, 2015 |
| Contact name |
Kaibo Duan |
| E-mail(s) |
duan_kaibo@immunol.a-star.edu.sg
|
| Organization name |
Singapore Immunology Network
|
| Street address |
8A Biomedical Grove, Immunos Building, Level 4
|
| City |
Singapore |
| ZIP/Postal code |
138648 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE60317 |
Transcriptome profile of human dermal macrophages and monocyte-derived macrophages |
|
| Relations |
| Reanalyzed by |
GSE74316 |
