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Status
Public on Dec 01, 2014
Title
C6
Sample type
RNA
Source name
control
Organism
Homo sapiens
Characteristics
cell type: H tert Fibroblasts
Treatment protocol
HTert-immortalized fibroblasts from C1, C6, C16, P1, P2 and P3 were treated with 1000U of IFN-α2b for 12 h, washed with PBS and left to rest for 36 h, after which relative mRNA levels were assessed
Extracted molecule
total RNA
Extraction protocol
RNAeasy kits from Qiagen
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA.
Hybridization protocol
Standard Affymetrix protocol was perfomed
Scan protocol
GeneChips were scanned using standard GeneArray Scanner.
Description
C6
Data processing
Affymetrix Console and TAC
Submission date
Aug 12, 2014
Last update date
Dec 01, 2014
Contact name
Dusan Bogunovic
E-mail(s)
dusan.bogunovic@mssm.edu
Organization name
Icahn School of Medicine at Mount Siani
Department
Microbiology
Street address
1468 Madison Avenue,
City
New York
State/province
New York
ZIP/Postal code
10029
Country
USA
Platform ID
GPL17586
Series (1)
GSE60359
ISG15 in regulation of interferon responses
Data table header descriptions
ID_REF
VALUE
log2 rma-gene-full
Data table
ID_REF
VALUE
TC01000001.hg.1
6.80429
TC01000002.hg.1
5.76356
TC01000003.hg.1
3.05507
TC01000004.hg.1
3.18188
TC01000005.hg.1
7.8688
TC01000006.hg.1
11.7941
TC01000007.hg.1
11.6093
TC01000008.hg.1
5.8482
TC01000009.hg.1
2.57435
TC01000010.hg.1
4.26325
TC01000011.hg.1
6.58349
TC01000012.hg.1
4.20905
TC01000013.hg.1
9.83956
TC01000014.hg.1
3.19603
TC01000015.hg.1
5.54353
TC01000016.hg.1
4.20884
TC01000017.hg.1
6.30417
TC01000018.hg.1
8.11717
TC01000019.hg.1
6.70817
TC01000020.hg.1
7.4134
Total number of rows: 70523 Table truncated, full table size 1655 Kbytes .Supplementary file
Size
Download
File type/resource
GSM1474240_4_HTA_2.0_Dusan_08132013_HTA-2_0_.CEL.gz
25.8 Mb
(ftp) (http)
CEL
GSM1474240_4_HTA_2.0_Dusan_08132013_HTA-2_0_.RMA.rma-gene-full.chp.gz
457.4 Kb
(ftp) (http)
CHP
Processed data included within Sample table
Processed data provided as supplementary file