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Status
Public on Dec 01, 2014
Title
P1 plus IFNa
Sample type
RNA
Source name
ISG15 deficient patient plus IFNa
Organism
Homo sapiens Characteristics
cell type: H tert Fibroblasts
Treatment protocol
HTert-immortalized fibroblasts from C1, C6, C16, P1, P2 and P3 were treated with 1000U of IFN-α2b for 12 h, washed with PBS and left to rest for 36 h, after which relative mRNA levels were assessed
Extracted molecule
total RNA
Extraction protocol
RNAeasy kits from Qiagen
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA.
Hybridization protocol
Standard Affymetrix protocol was perfomed
Scan protocol
GeneChips were scanned using standard GeneArray Scanner.
Description
P1 plus IFNa
Data processing
Affymetrix Console and TAC
Submission date
Aug 12, 2014
Last update date
Dec 01, 2014
Contact name
Dusan Bogunovic
E-mail(s)
dusan.bogunovic@mssm.edu Organization name
Icahn School of Medicine at Mount Siani
Department
Microbiology
Street address
1468 Madison Avenue,
City
New York
State/province
New York
ZIP/Postal code
10029
Country
USA
Platform ID
GPL17586 Series (1)
GSE60359 ISG15 in regulation of interferon responses
Data table header descriptions ID_REF VALUE log2 rma-gene-full
Data table ID_REF VALUE TC01000001.hg.1
6.18367
TC01000002.hg.1
5.61945
TC01000003.hg.1
3.24683
TC01000004.hg.1
2.95853
TC01000005.hg.1
8.3389
TC01000006.hg.1
12.2335
TC01000007.hg.1
12.0719
TC01000008.hg.1
5.96321
TC01000009.hg.1
2.51756
TC01000010.hg.1
4.31278
TC01000011.hg.1
6.70724
TC01000012.hg.1
4.17178
TC01000013.hg.1
9.67818
TC01000014.hg.1
3.22891
TC01000015.hg.1
5.53864
TC01000016.hg.1
4.27285
TC01000017.hg.1
6.94697
TC01000018.hg.1
8.13129
TC01000019.hg.1
6.71863
TC01000020.hg.1
7.06
70523 1655 Kbytes .Supplementary file Size Download File type/resource GSM1474248_14_HTA_2.0_Dusan_08132013_HTA-2_0_.CEL.gz
25.5 Mb
(ftp) (http) CEL
GSM1474248_14_HTA_2.0_Dusan_08132013_HTA-2_0_.RMA.rma-gene-full.chp.gz
457.4 Kb
(ftp) (http) CHP
Processed data included within Sample table Processed data provided as supplementary file
