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Status |
Public on Jul 01, 2015 |
Title |
Wild type VSMCs, static, biological rep2 |
Sample type |
RNA |
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Source name |
M_Ko2
|
Organism |
Mus musculus |
Characteristics |
genotype: WT cell type: vascular smooth muscle cells treatment: normal genetic background: C57BL/6J
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Treatment protocol |
Cells were stretched to 10% cyclic elongation for 6 hours at a frequency of 0.5 Hertz. Static, unstretched cells were used as a control.
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Growth protocol |
Primary mouse aortic vascular smooth muscle cells were grown on flexible membranes in presence of DMEM medium supplemented with 15% FCS in an incubator at 37 degree C and 5% CO2.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using a kit from Qiagen and tested for quality. sample were delivered for microarray analysis.
|
Label |
biotin
|
Label protocol |
Fifteen μl (corresponding to 5-10 μg total RNA) of each biotinylated cRNA were prepared according to the standard Affymetrix protocol.
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Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
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Scan protocol |
Affymetrix GeneArray Scanner3000
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Description |
Gene expression data from static wild type VSMCs
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Data processing |
The data were analyzed with JMP Genomics V. 6 from SAS. Gene expression profiling was performed using arrays of from Affymetrix. A Custom CDF with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary.
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Submission date |
Aug 15, 2014 |
Last update date |
Jul 01, 2015 |
Contact name |
Carsten Sticht |
Organization name |
University Heidelberg
|
Department |
ZMF
|
Street address |
Theodor-Kutzer-Ufer
|
City |
Mannheim |
ZIP/Postal code |
68169 |
Country |
Germany |
|
|
Platform ID |
GPL18615 |
Series (1) |
GSE60447 |
Stretch-dependent genes in vascular smooth muscle cells |
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