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Sample GSM1480253 Query DataSets for GSM1480253
Status Public on Jul 01, 2015
Title Wild type VSMCs, static, biological rep2
Sample type RNA
Source name M_Ko2
Organism Mus musculus
Characteristics genotype: WT
cell type: vascular smooth muscle cells
treatment: normal
genetic background: C57BL/6J
Treatment protocol Cells were stretched to 10% cyclic elongation for 6 hours at a frequency of 0.5 Hertz. Static, unstretched cells were used as a control.
Growth protocol Primary mouse aortic vascular smooth muscle cells were grown on flexible membranes in presence of DMEM medium supplemented with 15% FCS in an incubator at 37 degree C and 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using a kit from Qiagen and tested for quality. sample were delivered for microarray analysis.
Label biotin
Label protocol Fifteen μl (corresponding to 5-10 μg total RNA) of each biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description Gene expression data from static wild type VSMCs
Data processing The data were analyzed with JMP Genomics V. 6 from SAS. Gene expression profiling was performed using arrays of from Affymetrix. A Custom CDF with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary.
Submission date Aug 15, 2014
Last update date Jul 01, 2015
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
Platform ID GPL18615
Series (1)
GSE60447 Stretch-dependent genes in vascular smooth muscle cells

Data table header descriptions
VALUE RMA signal intensity

Data table
100008567_at 7.02441406
100009600_at 4.078125
100012_at 2.56054688
100017_at 6.375
100019_at 7.19042969
100034251_at 4.44726563
100034739_at 3.25878906
100034748_at 5.25097656
100036520_at 2.62841797
100036521_at 8.6015625
100036523_at 3.02001953
100036768_at 3.12890625
100037258_at 9.02148438
100037262_at 3.14111328
100037278_at 6.15429688
100038347_at 3.82568359
100038358_at 2.63183594
100038371_at 2.30810547
100038392_at 3.10839844
100038398_at 4.55957031

Total number of rows: 17502

Table truncated, full table size 344 Kbytes.

Supplementary file Size Download File type/resource
GSM1480253_Cattaruzza_030408_M-Ko2_Mou430_2.CEL.gz 5.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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