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Sample GSM1480254 Query DataSets for GSM1480254
Status Public on Jul 01, 2015
Title Wild type VSMCs, static, biological rep3
Sample type RNA
 
Source name M_Ko3
Organism Mus musculus
Characteristics genotype: WT
cell type: vascular smooth muscle cells
treatment: normal
genetic background: C57BL/6J
Treatment protocol Cells were stretched to 10% cyclic elongation for 6 hours at a frequency of 0.5 Hertz. Static, unstretched cells were used as a control.
Growth protocol Primary mouse aortic vascular smooth muscle cells were grown on flexible membranes in presence of DMEM medium supplemented with 15% FCS in an incubator at 37 degree C and 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using a kit from Qiagen and tested for quality. sample were delivered for microarray analysis.
Label biotin
Label protocol Fifteen μl (corresponding to 5-10 μg total RNA) of each biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description Gene expression data from static wild type VSMCs
Data processing The data were analyzed with JMP Genomics V. 6 from SAS. Gene expression profiling was performed using arrays of from Affymetrix. A Custom CDF with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary.
 
Submission date Aug 15, 2014
Last update date Jul 01, 2015
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL18615
Series (1)
GSE60447 Stretch-dependent genes in vascular smooth muscle cells

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
100008567_at 6.65136719
100009600_at 4.35839844
100012_at 2.34082031
100017_at 6.37597656
100019_at 6.91601563
100034251_at 7.515625
100034739_at 3.34033203
100034748_at 5.12011719
100036520_at 2.54248047
100036521_at 7.95800781
100036523_at 3.01953125
100036768_at 3.28710938
100037258_at 8.48046875
100037262_at 3.33300781
100037278_at 6.23730469
100038347_at 3.5703125
100038358_at 2.66699219
100038371_at 2.65234375
100038392_at 2.67333984
100038398_at 3.32763672

Total number of rows: 17502

Table truncated, full table size 344 Kbytes.




Supplementary file Size Download File type/resource
GSM1480254_Cattaruzza_030408_M-Ko3_Mou430_2.CEL.gz 5.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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