NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1480255 Query DataSets for GSM1480255
Status Public on Jul 01, 2015
Title Wild type VSMCs, stretched, biological rep1
Sample type RNA
 
Source name M_S1
Organism Mus musculus
Characteristics genotype: WT
cell type: vascular smooth muscle cells
treatment: stretched
genetic background: C57BL/6J
Treatment protocol Cells were stretched to 10% cyclic elongation for 6 hours at a frequency of 0.5 Hertz. Static, unstretched cells were used as a control.
Growth protocol Primary mouse aortic vascular smooth muscle cells were grown on flexible membranes in presence of DMEM medium supplemented with 15% FCS in an incubator at 37 degree C and 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using a kit from Qiagen and tested for quality. sample were delivered for microarray analysis.
Label biotin
Label protocol Fifteen μl (corresponding to 5-10 μg total RNA) of each biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description Gene expression data from stretched wild type VSMCs
Data processing The data were analyzed with JMP Genomics V. 6 from SAS. Gene expression profiling was performed using arrays of from Affymetrix. A Custom CDF with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary.
 
Submission date Aug 15, 2014
Last update date Jul 01, 2015
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL18615
Series (1)
GSE60447 Stretch-dependent genes in vascular smooth muscle cells

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
100008567_at 6.49023438
100009600_at 4.70605469
100012_at 2.49121094
100017_at 6.38476563
100019_at 6.81738281
100034251_at 7.54101563
100034739_at 3.09619141
100034748_at 5.37695313
100036520_at 2.89892578
100036521_at 8.13476563
100036523_at 3.48828125
100036768_at 3.03222656
100037258_at 8.60546875
100037262_at 3.03027344
100037278_at 6.38867188
100038347_at 3.74560547
100038358_at 2.67626953
100038371_at 2.71582031
100038392_at 2.734375
100038398_at 2.93261719

Total number of rows: 17502

Table truncated, full table size 344 Kbytes.




Supplementary file Size Download File type/resource
GSM1480255_Cattaruzza_030408_M-S1_Mou430_2.CEL.gz 5.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap