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Sample GSM1480258 Query DataSets for GSM1480258
Status Public on Jul 01, 2015
Title Zyxin-null VSMCs, stretched, biological rep1
Sample type RNA
 
Source name M_Sko01
Organism Mus musculus
Characteristics genotype: zyxin KO
cell type: vascular smooth muscle cells
treatment: stretched
genetic background: C57BL/6J
Treatment protocol Cells were stretched to 10% cyclic elongation for 6 hours at a frequency of 0.5 Hertz. Static, unstretched cells were used as a control.
Growth protocol Primary mouse aortic vascular smooth muscle cells were grown on flexible membranes in presence of DMEM medium supplemented with 15% FCS in an incubator at 37 degree C and 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using a kit from Qiagen and tested for quality. sample were delivered for microarray analysis.
Label biotin
Label protocol Fifteen μl (corresponding to 5-10 μg total RNA) of each biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description Gene expression data from stretched Zyxin-null VSMCs
Data processing The data were analyzed with JMP Genomics V. 6 from SAS. Gene expression profiling was performed using arrays of from Affymetrix. A Custom CDF with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary.
 
Submission date Aug 15, 2014
Last update date Jul 01, 2015
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL18615
Series (1)
GSE60447 Stretch-dependent genes in vascular smooth muscle cells

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
100008567_at 6.46191406
100009600_at 4.56640625
100012_at 2.34375
100017_at 7.08691406
100019_at 7.80175781
100034251_at 4.953125
100034739_at 3.11279297
100034748_at 5.14941406
100036520_at 2.55908203
100036521_at 7.72363281
100036523_at 3.04443359
100036768_at 2.91162109
100037258_at 9.44726563
100037262_at 3.16210938
100037278_at 5.65820313
100038347_at 3.75390625
100038358_at 2.58300781
100038371_at 2.30908203
100038392_at 2.99755859
100038398_at 4.01953125

Total number of rows: 17502

Table truncated, full table size 344 Kbytes.




Supplementary file Size Download File type/resource
GSM1480258_Cattaruzza_030408_M-Sko01_Mou430_2.CEL.gz 5.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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