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Sample GSM1480259 Query DataSets for GSM1480259
Status Public on Jul 01, 2015
Title Zyxin-null VSMCs, stretched, biological rep2
Sample type RNA
 
Source name M_Sko02
Organism Mus musculus
Characteristics genotype: zyxin KO
cell type: vascular smooth muscle cells
treatment: stretched
genetic background: C57BL/6J
Treatment protocol Cells were stretched to 10% cyclic elongation for 6 hours at a frequency of 0.5 Hertz. Static, unstretched cells were used as a control.
Growth protocol Primary mouse aortic vascular smooth muscle cells were grown on flexible membranes in presence of DMEM medium supplemented with 15% FCS in an incubator at 37 degree C and 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using a kit from Qiagen and tested for quality. sample were delivered for microarray analysis.
Label biotin
Label protocol Fifteen μl (corresponding to 5-10 μg total RNA) of each biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description Gene expression data from stretched Zyxin-null VSMCs
Data processing The data were analyzed with JMP Genomics V. 6 from SAS. Gene expression profiling was performed using arrays of from Affymetrix. A Custom CDF with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary.
 
Submission date Aug 15, 2014
Last update date Jul 01, 2015
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL18615
Series (1)
GSE60447 Stretch-dependent genes in vascular smooth muscle cells

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
100008567_at 6.23144531
100009600_at 4.59863281
100012_at 2.38818359
100017_at 7.1875
100019_at 7.89257813
100034251_at 4.98535156
100034739_at 3.1328125
100034748_at 5.04492188
100036520_at 2.35107422
100036521_at 7.96289063
100036523_at 3.10302734
100036768_at 2.82324219
100037258_at 9.53320313
100037262_at 3.42675781
100037278_at 5.66699219
100038347_at 3.75585938
100038358_at 2.71630859
100038371_at 2.76318359
100038392_at 3.01708984
100038398_at 4.15234375

Total number of rows: 17502

Table truncated, full table size 344 Kbytes.




Supplementary file Size Download File type/resource
GSM1480259_Cattaruzza_030408_M-Sko02_Mou430_2.CEL.gz 5.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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