|
| Status |
Public on Jul 01, 2015 |
| Title |
Zyxin-null VSMCs, stretched, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
M_Sko03
|
| Organism |
Mus musculus |
| Characteristics |
genotype: zyxin KO
cell type: vascular smooth muscle cells
treatment: stretched
genetic background: C57BL/6J
|
| Treatment protocol |
Cells were stretched to 10% cyclic elongation for 6 hours at a frequency of 0.5 Hertz. Static, unstretched cells were used as a control.
|
| Growth protocol |
Primary mouse aortic vascular smooth muscle cells were grown on flexible membranes in presence of DMEM medium supplemented with 15% FCS in an incubator at 37 degree C and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using a kit from Qiagen and tested for quality. sample were delivered for microarray analysis.
|
| Label |
biotin
|
| Label protocol |
Fifteen μl (corresponding to 5-10 μg total RNA) of each biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
Gene expression data from stretched Zyxin-null VSMCs
|
| Data processing |
The data were analyzed with JMP Genomics V. 6 from SAS. Gene expression profiling was performed using arrays of from Affymetrix. A Custom CDF with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary.
|
| |
|
| Submission date |
Aug 15, 2014 |
| Last update date |
Jul 01, 2015 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18615 |
| Series (1) |
| GSE60447 |
Stretch-dependent genes in vascular smooth muscle cells |
|
