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Sample GSM148077 Query DataSets for GSM148077
Status Public on Dec 06, 2006
Title ß-catenin transduced LSK day 3
Sample type RNA
 
Source name β-catenin transduced linnegSca-1posc-kitpos cells (after 3 days of retroviral transduction)
Organism Mus musculus
Characteristics genetic modification:
tissue: Bone marrow
Growth protocol IMDM-Medium + 10% FCS + 1% Pen/Strep + 20mM Hepes + Non Essential Amino Acids + Sodium Pyruvate + Zytokine (IL-3, SCF, Flt3, IL-11)
Extracted molecule total RNA
Extraction protocol Rneasy
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GCS 3000.
Description ß-catenin transduced LSK day 3
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
 
Submission date Dec 04, 2006
Last update date Aug 28, 2018
Contact name Robert Geffers
E-mail(s) robert.geffers@helmholtz-hzi.de
Phone +49 531-6181-3058
Organization name HCI - Helmholtz Centre for Infection Research
Department Dep. Molecular Bacteriology
Lab Genome Analytics
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
 
Platform ID GPL1261
Series (1)
GSE6424 ß-catenin Plays a Dual Role in the Generation and Maintenance of Immortalized Hematopoietic Stem Cells
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 163.5 P 0.009337
AFFX-BioB-M_at 181.2 P 0.00039
AFFX-BioB-3_at 200.5 P 0.009337
AFFX-BioC-5_at 803.3 P 0.000052
AFFX-BioC-3_at 638.2 P 0.00006
AFFX-BioDn-5_at 1273.7 P 0.000044
AFFX-BioDn-3_at 4365.9 P 0.000044
AFFX-CreX-5_at 3478 P 0.000044
AFFX-CreX-3_at 8971.8 P 0.000044
AFFX-DapX-5_at 7.2 A 0.672921
AFFX-DapX-M_at 40.7 A 0.470241
AFFX-DapX-3_at 36.6 A 0.368427
AFFX-LysX-5_at 12641.9 P 0.000044
AFFX-LysX-M_at 25846.3 P 0.000044
AFFX-LysX-3_at 18227.2 P 0.000044
AFFX-PheX-5_at 4.1 A 0.927318
AFFX-PheX-M_at 8.8 A 0.60308
AFFX-PheX-3_at 9.9 A 0.724854
AFFX-ThrX-5_at 5880.7 P 0.00007
AFFX-ThrX-M_at 6874.5 P 0.000044

Total number of rows: 45101

Table truncated, full table size 1193 Kbytes.




Supplementary file Size Download File type/resource
GSM148077.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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