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Sample GSM148078 Query DataSets for GSM148078
Status Public on Dec 06, 2006
Title eGFP transduced LSK day 3
Sample type RNA
 
Source name eGFP transduced linnegSca-1posc-kitpos cells (after 3 days of retroviral transduction)
Organism Mus musculus
Characteristics genetic modification:
tissue: Bone marrow
Growth protocol IMDM-Medium + 10% FCS + 1% Pen/Strep + 20mM Hepes + Non Essential Amino Acids + Sodium Pyruvate + Zytokine (IL-3, SCF, Flt3, IL-11)
Extracted molecule total RNA
Extraction protocol Rneasy
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GCS 3000.
Description eGFP transduced LSK day 3
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
 
Submission date Dec 04, 2006
Last update date Aug 28, 2018
Contact name Robert Geffers
E-mail(s) robert.geffers@helmholtz-hzi.de
Phone +49 531-6181-3058
Organization name HCI - Helmholtz Centre for Infection Research
Department Dep. Molecular Bacteriology
Lab Genome Analytics
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
 
Platform ID GPL1261
Series (1)
GSE6424 ß-catenin Plays a Dual Role in the Generation and Maintenance of Immortalized Hematopoietic Stem Cells
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 216.9 P 0.00844
AFFX-BioB-M_at 319.7 P 0.00141
AFFX-BioB-3_at 378.7 P 0.001796
AFFX-BioC-5_at 1020.1 P 0.000095
AFFX-BioC-3_at 881.2 P 0.00011
AFFX-BioDn-5_at 1557 P 0.00007
AFFX-BioDn-3_at 6445.6 P 0.000044
AFFX-CreX-5_at 4674.2 P 0.000044
AFFX-CreX-3_at 12437.5 P 0.000044
AFFX-DapX-5_at 3.7 A 0.910522
AFFX-DapX-M_at 60.2 A 0.175328
AFFX-DapX-3_at 7.4 A 0.843268
AFFX-LysX-5_at 12111.6 P 0.000044
AFFX-LysX-M_at 29119.6 P 0.000044
AFFX-LysX-3_at 21726.8 P 0.000044
AFFX-PheX-5_at 4.5 A 0.737173
AFFX-PheX-M_at 19.4 A 0.52976
AFFX-PheX-3_at 24 A 0.559354
AFFX-ThrX-5_at 899.8 P 0.000095
AFFX-ThrX-M_at 1997.7 P 0.000044

Total number of rows: 45101

Table truncated, full table size 1193 Kbytes.




Supplementary file Size Download File type/resource
GSM148078.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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