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Sample GSM148079 Query DataSets for GSM148079
Status Public on Dec 06, 2006
Title DKmix cells day 50
Sample type RNA
 
Source name ß-catenin transduced linnegSca-1posc-kitpos cells cultured for 50 days
Organism Mus musculus
Characteristics genetic modification:
tissue: Bone marrow
Growth protocol IMDM-Medium + 10% FCS + 1% Pen/Strep + 20mM Hepes + Non Essential Amino Acids + Sodium Pyruvate + Zytokine (IL-3, SCF, Flt3, IL-11)
Extracted molecule total RNA
Extraction protocol Rneasy
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GCS 3000.
Description DKmix cells day 50
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
 
Submission date Dec 04, 2006
Last update date Aug 28, 2018
Contact name Robert Geffers
E-mail(s) robert.geffers@helmholtz-hzi.de
Phone +49 531-6181-3058
Organization name HCI - Helmholtz Centre for Infection Research
Department Dep. Molecular Bacteriology
Lab Genome Analytics
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
 
Platform ID GPL1261
Series (1)
GSE6424 ß-catenin Plays a Dual Role in the Generation and Maintenance of Immortalized Hematopoietic Stem Cells
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 207.1 P 0.010317
AFFX-BioB-M_at 509.5 P 0.000081
AFFX-BioB-3_at 509.8 P 0.000754
AFFX-BioC-5_at 1671 P 0.000052
AFFX-BioC-3_at 1217.7 P 0.00007
AFFX-BioDn-5_at 2697.3 P 0.00007
AFFX-BioDn-3_at 10343.6 P 0.000044
AFFX-CreX-5_at 7013.7 P 0.000044
AFFX-CreX-3_at 17020.7 P 0.000052
AFFX-DapX-5_at 13.1 A 0.883887
AFFX-DapX-M_at 5.3 A 0.949771
AFFX-DapX-3_at 50.4 A 0.51489
AFFX-LysX-5_at 4735.7 P 0.000044
AFFX-LysX-M_at 26625.3 P 0.000044
AFFX-LysX-3_at 32402.9 P 0.000052
AFFX-PheX-5_at 4.3 A 0.876428
AFFX-PheX-M_at 10.2 A 0.876428
AFFX-PheX-3_at 40.4 A 0.712257
AFFX-ThrX-5_at 1861.7 P 0.00007
AFFX-ThrX-M_at 3215.9 P 0.000052

Total number of rows: 45101

Table truncated, full table size 1195 Kbytes.




Supplementary file Size Download File type/resource
GSM148079.CEL.gz 3.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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