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Sample GSM148080 Query DataSets for GSM148080
Status Public on Dec 06, 2006
Title DKmix cells day 300
Sample type RNA
 
Source name ß-catenin transduced linnegSca-1posc-kitpos cells cultured for 300 days (DKmix)
Organism Mus musculus
Characteristics genetic modification:
tissue: Bone marrow
Growth protocol IMDM-Medium + 10% FCS + 1% Pen/Strep + 20mM Hepes + Non Essential Amino Acids + Sodium Pyruvate + Zytokine (IL-3, SCF, Flt3, IL-11)
Extracted molecule total RNA
Extraction protocol Rneasy
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GCS 3000.
Description DKmix cells day 300
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
 
Submission date Dec 04, 2006
Last update date Aug 28, 2018
Contact name Robert Geffers
E-mail(s) robert.geffers@helmholtz-hzi.de
Phone +49 531-6181-3058
Organization name HCI - Helmholtz Centre for Infection Research
Department Dep. Molecular Bacteriology
Lab Genome Analytics
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
 
Platform ID GPL1261
Series (1)
GSE6424 ß-catenin Plays a Dual Role in the Generation and Maintenance of Immortalized Hematopoietic Stem Cells
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 265.1 P 0.000972
AFFX-BioB-M_at 599.3 P 0.000169
AFFX-BioB-3_at 591.4 P 0.00006
AFFX-BioC-5_at 1452.9 P 0.000044
AFFX-BioC-3_at 1628.1 P 0.000044
AFFX-BioDn-5_at 3111.5 P 0.000044
AFFX-BioDn-3_at 4871.9 P 0.000044
AFFX-CreX-5_at 5675.4 P 0.000044
AFFX-CreX-3_at 13818.2 P 0.000044
AFFX-DapX-5_at 6.2 A 0.617401
AFFX-DapX-M_at 8.9 A 0.52976
AFFX-DapX-3_at 6.9 A 0.645559
AFFX-LysX-5_at 10529.1 P 0.000044
AFFX-LysX-M_at 18219.3 P 0.000044
AFFX-LysX-3_at 15646.8 P 0.000044
AFFX-PheX-5_at 8.1 A 0.672921
AFFX-PheX-M_at 5.7 A 0.631562
AFFX-PheX-3_at 41.2 A 0.287743
AFFX-ThrX-5_at 5894 P 0.000052
AFFX-ThrX-M_at 7026.2 P 0.000044

Total number of rows: 45101

Table truncated, full table size 1193 Kbytes.




Supplementary file Size Download File type/resource
GSM148080.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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