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Sample GSM1483652 Query DataSets for GSM1483652
Status Public on Jul 24, 2020
Title Residual Lesion GFP Plus 2
Sample type RNA
 
Source name Residual Lesion GFP Plus
Organism Mus musculus
Characteristics strain: nu/nu
gender: Female
tissue: Isolated Orthotopic Residual Lesion Tumor Cells
cell genotype: MMTV-rtTA;TetO-HER2/neu
treatment: HER2/neu inhibition
time point: 28d after doxycycline withdrawal
Treatment protocol Doxycycline withdrawal was used in order to inactivate oncogene and lead to tumor regression
Growth protocol H2B-eGFP-labeled tumor cells were grown orthotopically in nu/nu mice on doxycycline
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Affymetrix Generic Hybridization
Scan protocol Arrays were scanned using the Affymetrix GeneArray Scanner
Data processing RMA using Brainarray custom CDF, mogene10st_Mm_ENTREZG; R 3.0.2
 
Submission date Aug 21, 2014
Last update date Jul 24, 2020
Contact name Lewis Chodosh
E-mail(s) chodosh@pennmedicine.upenn.edu
Phone (215) 898-1321
Organization name University of Pennsylvania
Department Cancer Biology
Lab Chodosh
Street address 421 Curie Blvd
City Philadelphia
State/province Pennsylvania
ZIP/Postal code 19104
Country USA
 
Platform ID GPL19103
Series (2)
GSE60609 In vivo isolated HER2/neu-Prim1 Primary, Residual, and Recurrent Tumor Cells, and Recurrent Tumor Stromal Cells
GSE60611 Tumor cells that survive oncogenic pathway inhibition exhibit cellular dormancy

Data table header descriptions
ID_REF
VALUE Normalized signal (log2 scale)

Data table
ID_REF VALUE
100008567_at 4.730656487
100009600_at 3.258472082
100009609_at 2.335738028
100009614_at 6.012665312
100012_at 3.364152782
100017_at 5.053662872
100019_at 5.440288274
100033459_at 3.879429077
100034251_at 3.87016723
100034748_at 5.118737159
100036520_at 4.811375162
100036521_at 3.852911398
100036523_at 6.360933789
100036537_at 4.996812414
100036768_at 4.048962921
100037258_at 9.480974423
100037260_at 5.776332967
100037262_at 4.547593341
100037278_at 5.326799346
100037396_at 3.964110472

Total number of rows: 21266

Table truncated, full table size 444 Kbytes.




Supplementary file Size Download File type/resource
GSM1483652_13_MoGene1ST_073012_MoGene-1_0-st-v1_.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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