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Sample GSM1483658 Query DataSets for GSM1483658
Status Public on Jul 24, 2020
Title Recurrent Tumor GFP Minus 2
Sample type RNA
 
Source name Recurrent Tumor GFP Minus
Organism Mus musculus
Characteristics strain: nu/nu
gender: Female
tissue: Isolated Orthotopic Recurrent Tumor Stromal Cells
cell genotype: MMTV-rtTA;TetO-HER2/neu
treatment: HER2/neu inhibition
time point: Recurrent Tumor
Treatment protocol Doxycycline withdrawal was used in order to inactivate oncogene and lead to tumor regression
Growth protocol H2B-eGFP-labeled tumor cells were grown orthotopically in nu/nu mice on doxycycline
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Affymetrix Generic Hybridization
Scan protocol Arrays were scanned using the Affymetrix GeneArray Scanner
Data processing RMA using Brainarray custom CDF, mogene10st_Mm_ENTREZG; R 3.0.2
 
Submission date Aug 21, 2014
Last update date Jul 24, 2020
Contact name Lewis Chodosh
E-mail(s) chodosh@pennmedicine.upenn.edu
Phone (215) 898-1321
Organization name University of Pennsylvania
Department Cancer Biology
Lab Chodosh
Street address 421 Curie Blvd
City Philadelphia
State/province Pennsylvania
ZIP/Postal code 19104
Country USA
 
Platform ID GPL19103
Series (2)
GSE60609 In vivo isolated HER2/neu-Prim1 Primary, Residual, and Recurrent Tumor Cells, and Recurrent Tumor Stromal Cells
GSE60611 Tumor cells that survive oncogenic pathway inhibition exhibit cellular dormancy

Data table header descriptions
ID_REF
VALUE Normalized signal (log2 scale)

Data table
ID_REF VALUE
100008567_at 4.695102508
100009600_at 3.190132392
100009609_at 2.28222707
100009614_at 5.896756967
100012_at 3.424369355
100017_at 5.415781916
100019_at 5.488411447
100033459_at 3.976659716
100034251_at 10.27181466
100034748_at 4.937910058
100036520_at 4.697683595
100036521_at 7.422502191
100036523_at 5.808163714
100036537_at 5.120133585
100036768_at 4.101611111
100037258_at 8.576604531
100037260_at 5.753513249
100037262_at 4.964766498
100037278_at 5.195094598
100037396_at 3.911166691

Total number of rows: 21266

Table truncated, full table size 444 Kbytes.




Supplementary file Size Download File type/resource
GSM1483658_26_MoGene1ST_073012_MoGene-1_0-st-v1_.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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