|
| Status |
Public on Jul 24, 2020 |
| Title |
Wnt1 GFP plus Residual Lesion 2 |
| Sample type |
RNA |
| |
|
| Source name |
Wnt1_gfp_ResidualLesion
|
| Organism |
Mus musculus |
| Characteristics |
strain: nu/nu
gender: Female
tissue: Isolated Orthotopic Residual Lesion Tumor Cells
cell genotype: MMTV-rtTA;TetO-Wnt1
treatment: Wnt1 inhibition
time point: 28d after doxycycline withdrawal
|
| Treatment protocol |
Doxycycline withdrawal was used in order to inactivate oncogene and lead to tumor regression
|
| Growth protocol |
H2B-eGFP-labeled tumor cells were grown orthotopically in nu/nu mice on doxycycline
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Affymetrix Generic Hybridization
|
| Scan protocol |
Arrays were scanned using the Affymetrix GeneArray Scanner
|
| Data processing |
RMA using Brainarray custom CDF, mogene20st_Mm_ENTREZG; R 3.0.2
|
| |
|
| Submission date |
Aug 21, 2014 |
| Last update date |
Jul 24, 2020 |
| Contact name |
Lewis Chodosh |
| E-mail(s) |
chodosh@pennmedicine.upenn.edu
|
| Phone |
(215) 898-1321
|
| Organization name |
University of Pennsylvania
|
| Department |
Cancer Biology
|
| Lab |
Chodosh
|
| Street address |
421 Curie Blvd
|
| City |
Philadelphia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL18802 |
| Series (2) |
| GSE60610 |
In vivo isolated Wnt1-Prim1 Primary, Residual, and Recurrent Tumor Cells |
| GSE60611 |
Tumor cells that survive oncogenic pathway inhibition exhibit cellular dormancy |
|
