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Sample GSM1483662 Query DataSets for GSM1483662
Status Public on Jul 24, 2020
Title Wnt1 GFP plus Residual Lesion 3
Sample type RNA
 
Source name Wnt1_gfp_ResidualLesion
Organism Mus musculus
Characteristics strain: nu/nu
gender: Female
tissue: Isolated Orthotopic Residual Lesion Tumor Cells
cell genotype: MMTV-rtTA;TetO-Wnt1
treatment: Wnt1 inhibition
time point: 28d after doxycycline withdrawal
Treatment protocol Doxycycline withdrawal was used in order to inactivate oncogene and lead to tumor regression
Growth protocol H2B-eGFP-labeled tumor cells were grown orthotopically in nu/nu mice on doxycycline
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Affymetrix Generic Hybridization
Scan protocol Arrays were scanned using the Affymetrix GeneArray Scanner
Data processing RMA using Brainarray custom CDF, mogene20st_Mm_ENTREZG; R 3.0.2
 
Submission date Aug 21, 2014
Last update date Jul 24, 2020
Contact name Dhruv K Pant
E-mail(s) dpant@pennmedicine.upenn.edu
Phone 9083381849
Organization name University of Pennsylvania
Department Cancer Biology
Lab Chodosh
Street address 421 Curie Blvd
City Philadelphia
State/province Pennsylvania
ZIP/Postal code 19104
Country USA
 
Platform ID GPL18802
Series (2)
GSE60610 In vivo isolated Wnt1-Prim1 Primary, Residual, and Recurrent Tumor Cells
GSE60611 Tumor cells that survive oncogenic pathway inhibition exhibit cellular dormancy

Data table header descriptions
ID_REF
VALUE Normalized signal (log2 scale)

Data table
ID_REF VALUE
100008567_at 3.842485879
100009600_at 3.194865115
100009609_at 1.927375178
100009614_at 2.682981798
100009664_at 2.921851786
100012_at 3.971509143
100017_at 5.182792328
100019_at 6.047987026
100033459_at 1.6753762
100034251_at 5.590303987
100034729_at 3.230516309
100034739_at 4.050824007
100034748_at 4.377544241
100036518_at 2.722350771
100036520_at 3.22763605
100036521_at 4.618153038
100036523_at 5.807136064
100036537_at 6.912481829
100036768_at 4.288891723
100037258_at 9.532688547

Total number of rows: 24479

Table truncated, full table size 515 Kbytes.




Supplementary file Size Download File type/resource
GSM1483662_L4_MoGene-2_0-st_.CEL.gz 8.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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