|
| Status |
Public on Apr 30, 2007 |
| Title |
Tumor from MYF-5 CRE/SSM2 mouse SSM5 |
| Sample type |
RNA |
| |
|
| Source name |
Tumor from MYF-5 CRE/SSM2 mouse
|
| Organism |
Mus musculus |
| Characteristics |
Adolescent mouse tumor sample
|
| Treatment protocol |
Tissue and tumor samples were excised and subjected to manual disruption prior to total RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions and was further purified using RNeasy kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
labeled target RNA was prepared from total tissue RNA using the One-Cycle Target Labeling Kit and Control Reagents
|
| |
|
| Hybridization protocol |
cRNA (15 ug) was fragmented following the Affymetrix protocol, added to 270 ul of hybridization buffer, and hybridized to the Affymetrix GeneChip Mouse Genome 430 2.0 arrays (P/N 900495)
|
| Scan protocol |
The arrays were scanned using an Affymetrix GeneChip Scanner 3000 enabled for High-Resolution Scanning, and the raw images were converted to .CEL files using Affymetrix GCOS software
|
| Description |
Gene expression data from SYT-SSX murine tumor
|
| Data processing |
The MAS5 algorithm was used to accomplish the mean scaling normalization and scaling to 500.
|
| |
|
| Submission date |
Dec 06, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jeffrey Hancock |
| E-mail(s) |
jhancock@hsc.utah.edu
|
| Organization name |
University of Utah
|
| Department |
Huntsman Cancer Institute
|
| Lab |
Lessnick Lab
|
| Street address |
2000 Circle of Hope Dr
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE6461 |
SYT-SSX murine synovial sarcoma model |
|
| Relations |
| Reanalyzed by |
GSE119085 |
