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Sample GSM148649 Query DataSets for GSM148649
Status Public on Dec 08, 2006
Title Adult male bladder J_238G
Sample type RNA
 
Source name bladder
Organism Mus musculus
Characteristics gene reported: SMGA/EGFP transgenic / PMID: 14713859
strain: FVB/N
sex: male
developmental stage: adult
theiler stage: 28
somite count: N/A
developmental landmark: Adult animal
Treatment protocol pool size: N/A
Pooled sample: no
Dissection Method: Gross dissection of entire bladder.
An adult SMGA/EGFP male was euthanized by carbon dioxide inhalation. Bladders were removed by dissection, rapidly frozen and stored in liquid nitrogen until RNA was extracted for gene expression analysis.
Extracted molecule total RNA
Extraction protocol Qiagen: RNeasy
Label Biotin
Label protocol Biotin-X-X-NHS (EPICENTRE Biotechnologies)
 
Hybridization protocol Affymetrix standard protocol
Amount labeled target hybridization to array: 10 ug
Scan protocol Affymetrix standard protocol
Description >> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter protocols
Rounds of amplification: 2
URL: http://gudmap.hgu.mrc.ac.uk/gudmap/pages/mic_submission.html?id=GUDMAP:7122
Data processing Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
 
Submission date Dec 06, 2006
Last update date Aug 28, 2018
Contact name GUDMAP Developers
E-mail(s) gudmap-db@gudmap.org
Phone +44 131 651 8500
Organization name IGMM MRC Human Genetics Unit
Lab GUDMAP Database Group
Street address Crewe Road
City Edinburgh
ZIP/Postal code EH4 2XU
Country United Kingdom
 
Platform ID GPL1261
Series (1)
GSE6466 Gene expression profiles of P1 and adult bladders isolated from SMGA (Actg2) transgenic mice. (GUDMAP Series ID: 11)
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1194.50 P 0.000195
AFFX-BioB-M_at 2042.03 P 0.000052
AFFX-BioB-3_at 1061.90 P 0.000052
AFFX-BioC-5_at 3513.59 P 0.000044
AFFX-BioC-3_at 4420.77 P 0.000044
AFFX-BioDn-5_at 8058.04 P 0.000044
AFFX-BioDn-3_at 14101.25 P 0.000044
AFFX-CreX-5_at 45930.25 P 0.000052
AFFX-CreX-3_at 52662.57 P 0.000044
AFFX-DapX-5_at 42.58 A 0.062929
AFFX-DapX-M_at 15.89 A 0.659352
AFFX-DapX-3_at 7.82 A 0.783476
AFFX-LysX-5_at 4.88 A 0.645547
AFFX-LysX-M_at 5.81 A 0.783496
AFFX-LysX-3_at 20.51 A 0.529760
AFFX-PheX-5_at 2.77 A 0.973889
AFFX-PheX-M_at 5.07 A 0.910522
AFFX-PheX-3_at 74.61 A 0.165861
AFFX-ThrX-5_at 65.72 A 0.195266
AFFX-ThrX-M_at 26.99 A 0.500000

Total number of rows: 45101

Table truncated, full table size 1291 Kbytes.




Supplementary file Size Download File type/resource
GSM148649.CEL.gz 3.8 Mb (ftp)(http) CEL

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