|
| Status |
Public on Nov 17, 2014 |
| Title |
Oa labrum;E |
| Sample type |
RNA |
| |
|
| Source name |
osteoarthritic labrum cells
|
| Organism |
Homo sapiens |
| Characteristics |
subject id: patient E
health condition: Osteoarthritic
tissue: acetabular labrum
cell type: labrum cells
culture age: 7 days
|
| Treatment protocol |
none
|
| Growth protocol |
Cultured for 7 days in alginate beads system in RPMI medium completed with serum and antibiotics
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylation was performed using GeneChip WT sense target labelling assay and reagents according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
After fragmentation cDNA was hybridized for 16h on 45°C on Human GeneChip Gene 1.0 ST array system.GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
Scanned by GeneChip Scanner 3000 7G
|
| Description |
SAMPLE 2
|
| Data processing |
standard affymetrix expression console software (v.1.1) was used for QC and to generated RMA
|
| |
|
| Submission date |
Aug 26, 2014 |
| Last update date |
Nov 17, 2014 |
| Contact name |
Dirk Elewaut |
| E-mail(s) |
dirk.elewaut@ugent.be
|
| Organization name |
Ghent University
|
| Department |
Rheumatology
|
| Street address |
de pintelaan 185
|
| City |
Ghent |
| State/province |
België |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE60762 |
Expression data of osteoarthritic and healthy human labrum cells |
|
