|
| Status |
Public on Dec 11, 2007 |
| Title |
Brenda_Richards_Hypothalamus_2choice diet_B6.CAST-17 congenics |
| Sample type |
RNA |
| |
|
| Source name |
Hypothalamus
|
| Organism |
Mus musculus |
| Characteristics |
STRAIN : B6.CAST-17 congenic mice strain
GENDER : MALE
TISSUE : HYPOTHALAMUS
DIET : 2-choice diet fed (Carbohydrate/protein and Fat/protein diet) for 24 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The tissues were removed quickly, frozen in liquid nitrogen and stored at -80 oC. Total RNA was isolated from harvested tissue using TRI-Reagent (Molecular Research Center Inc. Cincinnati, Ohio), and purified using Qiagen RNAeasy columns (Qiagen, Valencia, CA). RNA was stored at -80oC in RNAse free water. Quality and quantity of RNA was determined using Agilent Bioanalyzer (Agilent Technologies, Palo Alto, CA
|
| Label |
DIG-UTP
|
| Label protocol |
1 microgram of total RNA was used to transcribe DIG labeled cRNA using Applied Biosystems Chemiluminescent RT-IVT kit v2.0
|
| |
|
| Hybridization protocol |
Fifteen microgram of DIG labeled fragmented cRNA was used for microarray hybridization and performed according to the Applied Biosystems protocols
|
| Scan protocol |
Chemiluminescence detection, imaging, auto girding, and image analysis was done according to the Applied Biosystems protocols and the 1700 Chemiluminescent Microarray Analyzer Software v1.0.3
|
| Description |
Pooled hypothalamus samples from 6 B6.CAST-17 congenic mice fed with 2-choice diet paradigm (carbohydrate/protein and fat/protein diet).
|
| Data processing |
Signal intensities across microarrays were normalized using quantile-quantile normalization method (www.bioconductor.org)
|
| |
|
| Submission date |
Dec 07, 2006 |
| Last update date |
Jan 13, 2012 |
| Contact name |
Brenda Richards |
| E-mail(s) |
richarbk@pbrc.edu
|
| Organization name |
Pennington Biomedical Research Center
|
| Department |
Basic Science
|
| Lab |
Genetics of Eating Behavior
|
| Street address |
6400 Perkins Rd.
|
| City |
Baton Rouge |
| State/province |
LA |
| ZIP/Postal code |
70808 |
| Country |
USA |
| |
|
| Platform ID |
GPL2995 |
| Series (1) |
| GSE6507 |
Gene expression profiling in a congenic strain identifies candidate genes for nutrient intake QTL on mouse chromosome 17 |
|
