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Sample GSM1489838 Query DataSets for GSM1489838
Status Public on Dec 01, 2014
Title DC Donor 6 day 7 [30 Hy5TM]
Sample type RNA
 
Source name DC Donor 6 day 7
Organism Homo sapiens
Characteristics cell type: DC
donor: 6
time point: day7
Treatment protocol Cells were left untreated.
Growth protocol CD14+ monocytes were cultured at 3×106 cells/ml in either (a) RPMI 1640 medium supplemented with 10% FBS, 2 mmol/L L-glutamine, penicillin (100 U/ml), streptomycin (100 μg/ml), 1000U/ml IL-4, 500U/ml GM-CSF; or (b) DMEM medium supplemented with 10% FBS, 2 mmol/L L-glutamine, penicillin (100 U/ml), streptomycin (100 μg/ml), gentamicin (50ng/ml), 100ng/ml M-CSF. Media was replenished every 48 hours.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the miRNeasy kit (Qiagen) following manufacturer’s protocol. The RNA was quantitated using the NanoDrop (Thermo Scientific) and integrity assessed using the 2100 Bioanalyzer.
Label Hy5TM
Label protocol 250ng of total RNA was used as input for miRCURY LNATM microRNA Hi-Power Labeling Kit. Labelling was performed on an automated Tecan hybridization station.
 
Hybridization protocol Hybridisation was performed by Exiqon.
Scan protocol Scanning was performed by Exiqon.
Description The samples were labeled using the miRCURY LNATM microRNA Hi-Power Labeling Kit, Hy3TM/Hy5TM and hybridized on the miRCURY LNATM microRNA Array (7th Gen)
Data processing Data normalization was performed using Quantile normalization by Exiqon.
Quantile normalized signal
Fold change (Normalized Reads)
Expression analysis (ANOVA for Time - dendritic cells)
Expression analysis (ANOVA for Time - macrophages)
Expression analysis for DC_1h vs. Monocyte
Expression analysis for MP_1h vs. Monocyte
 
Submission date Aug 27, 2014
Last update date Dec 01, 2014
Contact name Jezrom Bokcaerin Self-Fordham
E-mail(s) jez.fordham@gmail.com
Phone 7735247565
Organization name UIC
Department Periodontics
Lab 461
Street address 801 S Paulina
City Chicago
State/province IL
ZIP/Postal code 60612
Country USA
 
Platform ID GPL19128
Series (1)
GSE60839 miRCURY LNATM microRNA Array (7th Gen) profiling of monocyte-derived macrophages and monocyte-derived dendritic cells.

Supplementary file Size Download File type/resource
GSM1489838_1_Exiqon_19708341_S01.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data are available on Series record

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