BJ neonatal foreskin primary fibroblasts were cultured in Ham's F10 medium supplemented with 10% fetal bovine serum, 100 U/ml penicillin, and 100 ug/ml streptomycin. The cells were transduced with TERT cDNA at population doubling PD=63. The population doubling of TERT expressing cells chosen for SAGE library construction was PD=110. The cells were ~80% confluent at the time of RNA isolation.
