|
| Status |
Public on Dec 06, 2007 |
| Title |
6 month old adult male littermate control +/Y, biol rep2 |
| Sample type |
RNA |
| |
|
| Source name |
microdissected olfactory epithelium
|
| Organism |
Mus musculus |
| Characteristics |
Genotype: +/Y; strain B6CBACa, male, 6 months old, olfactory epithelium
|
| Treatment protocol |
Following euthanasia with carbon dioxide, the olfactory epithelium was quickly microdissected, weighed, and snap-frozen in liquid nitrogen.
|
| Growth protocol |
The mice were purchased from Jackson Laboratories (Bar Harbor, ME) at 13 weeks of age and were maintained in an animal facility at the University of Kentucky Department of Laboratory Animal Research (DLAR) until they reached six months of age. At the DLAR, they were kept on a 12 hr light:dark cycle in Bioclean units with sterile-filtered air and provided food and water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Snap-frozen microdissected olfactory epithelium was pulverized in TRI Reagent (Sigma-Aldrich, St. Louis, MO) and processed through a QIAshredder column (Qiagen, Valencia, CA). The total RNA was further purified using the Qiagen RNeasy Mini-Kit according to the manufacturer’s protocol. Total RNA yield and purity was assessed with a spectrophotometer and with the model 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA); all samples had A260/A280 ratios of 1.9–2.2 and showed two sharp peaks corresponding to 18S and 28S RNA on the Bioanalyzer electropherograms.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 13.33 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array . GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS 3000 7G scanner
|
| Description |
Gene expression data from olfactory epithelium of littermate control mice (+/Y)
|
| Data processing |
The data were analyzed with Affymetrix MicroArray Suite 5.0 using Affymetrix default analysis settings. A threshold value of 1,500 was used to normalize the data across all the microarrays.
|
| |
|
| Submission date |
Dec 08, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Marilyn L. Getchell |
| E-mail(s) |
mgetch@uky.edu
|
| Organization name |
University of Kentucky
|
| Department |
Anatomy and Neurobiology
|
| Lab |
Sanders-Brown Center on Aging
|
| Street address |
800 South Limestone St.
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40536-0230 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE6485 |
Expression data from olfactory epithelium of Harlequin mutant mice compared to littermate controls |
|
| Relations |
| Reanalyzed by |
GSE119085 |
