|
| Status |
Public on Nov 21, 2007 |
| Title |
Long-term hematopoietic stem cells LT-HSC_SPKSL_2 |
| Sample type |
RNA |
| |
|
| Source name |
LT-HSC isolated via Flow cytometry
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57Bl/6, Gender: female, Age: 12-14 weeks, Pooled samples: 4
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
25,000- 50,000 cells were FACS sorted, RNA was isolated with RNAqueous Kit (Ambion) per manufacture's protocol, DNAse treated with DNAse1, phenol:chloroform:isoamyl alcohol extracted, and amplified through two rounds of in vitro transcription using MessageAmp (Ambion) per manufacture's protocol
|
| Label |
Biotin
|
| Label protocol |
Second round of MessageAmp(Ambion) amplification was biotinylated with bio-CTP and bio-UTP per manufature's protocol.
|
| |
|
| Hybridization protocol |
Standard protocol from manufacturer
|
| Scan protocol |
Hardware: GeneChip¨ Scanner 3000
|
| Description |
not applicable
|
| Data processing |
Normalization was done with the GCRMA package from Bioconductor
|
| |
|
| Submission date |
Dec 11, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Nathan C Boles |
| Organization name |
Baylor College of Medicine
|
| Department |
Pediatrics
|
| Lab |
Goodell
|
| Street address |
One Baylor Plaza, N1030
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE6506 |
Hematopoietic Fingerprints: an expression database of stem cells and their progeny |
|
| Relations |
| Reanalyzed by |
GSE119085 |
