|
| Status |
Public on Sep 06, 2014 |
| Title |
Pancreatic_tumors_of_PatientB2 |
| Sample type |
RNA |
| |
|
| Source name |
Pancreatic tumors of patient B2 without PCA-DM
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: pancreas
disease status: pancreatic tumor
gender: female
age: 60
fasting blood glucose(mmol/l): 4.5
|
| Treatment protocol |
Ethical approval for the study was granted from Sun Yat-sen Memorial Hospital Ethics Committee and written informed consent from each subject was obtained before commencing. Tissue samples were obtained from the surgery of patients. The resected tissues were collected, incubated in the liquid nitrogen immediately after the surgical resection, and stored at -80℃ for use.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was extracted by Trizol Reagent and cleaned up with Baseline-ZERO DNase(EPICENTR). mRNAs were purified by removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre).
|
| Label |
Cy3
|
| Label protocol |
mRNA of high quality was fragmented and then labeled with Quick Amp Labeling Kit(One-Color, Cy3, Agilent).
|
| |
|
| Hybridization protocol |
After purification, the labeled RNAs were hybridized with probes on the Hybridization Chamber gasket slides (a slide contained eight microarrays, Agilent,Arraystar Human LncRNA Array v3.0).
|
| Scan protocol |
The slides were washed carefully and scanned with Agilent Microarray Scanner G2505C.
|
| Description |
Gene expression of pancreatic tumors without PCA-DM
|
| Data processing |
The raw data was analyzed with the software Agilent Feature Extraction (Agilent Technology, version 11.0.1.1) and and subsequent data processing were performed using the GeneSpring GX v12.0 software package (Agilent Technologies).
|
| |
|
| Submission date |
Sep 05, 2014 |
| Last update date |
Sep 06, 2014 |
| Contact name |
Wenchao Gao |
| E-mail(s) |
gwc19880401@163.com
|
| Phone |
+8613570489101
|
| Organization name |
Sun Yat-sen Memorial Hospital of Sun Yat-sen University
|
| Department |
Department of Hepatobiliary Surgery
|
| Lab |
Chen Rufu's Lab
|
| Street address |
N.O. 107, Yuanjiang Road
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510120 |
| Country |
China |
| |
|
| Platform ID |
GPL16956 |
| Series (1) |
| GSE61166 |
Global mRNA/LncRNA expression analysis of pancreatic tumors causing type3C Diabetes Mellitus |
|
