|
| Status |
Public on Sep 11, 2014 |
| Title |
Collagen gel w/o PDGF-BB |
| Sample type |
RNA |
| |
|
| Source name |
HGF cells in gel w/o growth factor
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: gingival fibroblasts
scaffold: gel
growth factor: w/o PDGF-BB
|
| Treatment protocol |
Collagen scaffolds and gel (w/ and w/o PDGF-BB) was overlaid with HGF cells in BME and harvested after 1-14 days for RNA extraction
|
| Growth protocol |
HGF cells were suspended in BME with 1% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol method of RNA extraction was performed to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Scanner 3000 7G.
|
| Data processing |
The data were analyzed with Expression Console.
|
| |
|
| Submission date |
Sep 10, 2014 |
| Last update date |
Sep 11, 2014 |
| Contact name |
William Giannobile |
| E-mail(s) |
wgiannob@umich.edu
|
| Organization name |
University of Michigan
|
| Department |
Periodontics and Oral Medicine
|
| Street address |
1011 N. University Ave.
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE61314 |
Cell population kinetics of collagen scaffolds in ex vivo oral wound repair |
|
