NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM150339 Query DataSets for GSM150339
Status Public on Aug 01, 2008
Title MMT + si pea3 sample 1
Sample type RNA
 
Source name MMT
Organism Mus musculus
Characteristics ATCC CCL-51
Tissue: mammary gland
Disease: tumor
Biomaterial provider ATCC
Treatment protocol Transfection by siRNA
Growth protocol MMT were cultured on culture flasks (Becton Dickinson) in DMEM (Gibco, Invitrogen) supplemented with 10% (v/v) fetal calf serum (FCS, Gibco, Invitrogen), gentamycin (100 IU/ml) and non-essential amino acids (Gibco, Invitrogen)
Extracted molecule total RNA
Extraction protocol total RNA was isolated from MMT cells using RNeasy kit (Qiagen) according to the manufacturer.
Label digoxygenin
Label protocol 1µg were subjected to RT-IVT amplification and labeling according to the manufacturer (applied Biosystems, prodNo 4339628)
 
Hybridization protocol Labeled cRNAs were hybridized according to the supplied protocols (applied Biosystems, ProdNo 4346875)
Scan protocol Applied Biosystems Expression Array System Software v1.1.1. (ProdNo: 4364137) has been used to acquire the chemiluminescence and fluorescence images and primary data analysis.
Description Transcriptome profiles were acquired using novel Applied Biosystems AB1700 technology. All experimental data referred to as MGS V1.0 (Mouse Genome Survey Version 1, ProdNo: 4337467) were generated on the Applied Biosystems AB1700 transcriptome platform (ProdNo: 4338036). These arrays contain probes for 28,218 validated mouse genes.
Data processing Applied Biosystems Expression Array System Software v1.1.1.
 
Submission date Dec 14, 2006
Last update date Jul 29, 2008
Contact name Anne Chotteau
E-mail(s) anne.chotteau@ibl.fr
Phone 0033320871128
Fax 0033320871111
Organization name CNRS UMR 8161
Lab Institut de Biologie de Lille
Street address 1 rue du professeur calmette
City Lille
ZIP/Postal code 59021
Country France
 
Platform ID GPL2995
Series (1)
GSE6588 Target genes associated to siRNA mediated repression of Pea3 and Erm transcription factors in mammary cancerous cells

Data table header descriptions
ID_REF
VALUE same as UNF_VALUE but with flagged values removed
CV noise-background signal
Flags value > 4096 indicates failed measurement
UNF_VALUE assay normalized signal

Data table
ID_REF VALUE CV Flags UNF_VALUE
297784 559.43 0.06 0 559.43
297907 0.77 1.85 1 0.77
297912 28.46 0.06 0 28.46
297935 0.89 0.22 0 0.89
297990 4.59 0.13 0 4.59
297993 0.63 1.48 1 0.63
298000 96.67 0.04 0 96.67
298038 0.37 0.63 1 0.37
298121 0.60 0.68 1 0.60
298130 5.80 1.32 1 5.80
298143 3.90 0.11 0 3.90
298150 0.35 2.80 1 0.35
298151 0.84 0.76 0 0.84
298155 0.50 0.39 1 0.50
298165 4.29 0.11 0 4.29
298174 15.66 0.06 0 15.66
298188 0.43 2.41 1 0.43
298200 980.29 0.06 0 980.29
298246 0.82 4.76 1 0.82
298248 0.48 0.91 1 0.48

Total number of rows: 33012

Table truncated, full table size 797 Kbytes.




Supplementary file Size Download File type/resource
GSM150339.txt.gz 222.1 Kb (ftp)(http) TXT

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap