|
| Status |
Public on Mar 01, 2015 |
| Title |
iKOPGC-1β 6 |
| Sample type |
RNA |
| |
|
| Source name |
mouse ileum 6
|
| Organism |
Mus musculus |
| Characteristics |
strain: c57/Blk6
age: 3-4 months old
tissue: ileum
genotype/variation: iKOPGC-1β
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Qiazol reagent, following manufacturer's instructions. Quality control was performed with Biorad Experion.
|
| Label |
biotin
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
The data were normalised using quantile normalization (background was not subtracted) with Illumina Genome Studio Software; items with detection p values >0.001 or AVG Signal <200 were excluded. Data were analyzed with Genome Studio Software (Differential Expression Analysis with Mann-Whitney error model plus False Discovery Rate, FDR).
|
| |
|
| Submission date |
Sep 22, 2014 |
| Last update date |
Mar 01, 2015 |
| Contact name |
Elena Bellafante |
| E-mail(s) |
elena.bellafante@kcl.ac.uk
|
| Organization name |
KCL
|
| Department |
Women's Health
|
| Lab |
Fetal and Maternal Disease
|
| Street address |
Guy's Campus
|
| City |
London |
| ZIP/Postal code |
SE11UL |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE61640 |
Genome-wide analysis expression of ileum samples from PGC-1β fl/? and iKOPGC-1β |
| GSE61643 |
PGC-1β Promotes Enterocyte Lifespan and Tumorigenesis in the Intestine |
|
