|
| Status |
Public on Nov 20, 2007 |
| Title |
human_intracranial_artery_4 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA from human intracranial artery, non-aneurysm
|
| Organism |
Homo sapiens |
| Characteristics |
Tissue: Intracranial artery (non-aneurysm)
Source Disease Status: non-aneurysm
Individual/Pool: Individual
Age: 23 years
Gender: Male
Vessel: internal carotid artery and primary branch middle cerebral artery
Ethnicity: African American
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Guanidinium isothiocyanate, cesium chloride centrifugation (Sambrook Molecular Cloning: a laboratory manual)
|
| Label |
biotin, streptavidin-Cy3
|
| Label protocol |
153 ng of total RNA was amplified and labeled using the Illumina TotalPrep RNA Amplification Kit (Cat #IL1791), manual version 0606
|
| |
|
| Hybridization protocol |
1.5 micrograms of labeled cRNA was used for hybridization, and microarrays were washed and stained using standard Illumina BeadChip 6 X 2 protocol, Doc. # 11176837 Rev. E
|
| Scan protocol |
Scanned on Illumina BeadStation 500GX using standard Illumina protocol
|
| Description |
Intracranial arteries obtained from autopsy for mRNA expression analysis
|
| Data processing |
Cubic spline normalization using Illumina BeadStudio Version 1.5.0.34
|
| |
|
| Submission date |
Dec 17, 2006 |
| Last update date |
Nov 20, 2007 |
| Contact name |
Gerard Tromp |
| E-mail(s) |
gerard.tromp@sanger.med.wayne.edu
|
| Phone |
313-577-8773
|
| Fax |
313-577-5218
|
| Organization name |
Wayne State University School of Medicine
|
| Department |
Center for Molecular Medicine and Genetics
|
| Street address |
3309 Scott Hall, 540 East Canfield Ave
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48201 |
| Country |
USA |
| |
|
| Platform ID |
GPL2507 |
| Series (2) |
| GSE6551 |
Expression data from intracranial arteries and intracranial aneurysms |
| GSE7471 |
Gene Expression Profiles in Intracranial Arteries and Abdominal Aortae |
|
