|
| Status |
Public on Oct 03, 2014 |
| Title |
ob/ob hypothalamus, rep4 |
| Sample type |
RNA |
| |
|
| Source name |
ob/ob hypothalamus
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
genotype: ob/ob
gender: male
age: 9 weeks old
tissue: brain hypothalamus
|
| Treatment protocol |
For STZ-induced diabetes experiments, mice were treated with a single intraperitoneal (i.p.) injection (200 µg/g body weight) of STZ (Sigma). Control mice were injected with the same volume of vehicle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using an RNeasy kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Double-stranded cDNA synthesis was reverse-transcribed from 10 µg of isolated RNA from each hypothalamus by using the SuperScript (Invitrogen) with an oligo(dT) primer containing a T7 RNA polymerase promoter site. Double-stranded cDNA was purified with Phase Lock Gel (Eppendorf). Biotin-labeled cRNA was transcribed by using a BioArray RNA transcript labeling kit (Enzo).
|
| |
|
| Hybridization protocol |
A hybridization mixture containing 15 µg of biotinylated cRNA, adjusted for possible carryover of residual total RNA, was prepared and hybridized to the arrays
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Data processing |
Intensity values were quantified by using MAS 5.0 software (Affymetrix)
|
| |
|
| Submission date |
Oct 02, 2014 |
| Last update date |
Oct 03, 2014 |
| Contact name |
Jonathan M Dreyfuss |
| Organization name |
Joslin Diabetes Center
|
| Department |
Bioinformatics & Biostatistics Core
|
| Street address |
1 Joslin Pl
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE62013 |
Diabetes and Insulin in Regulation of Brain Cholesterol Metabolism |
|
