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Status |
Public on May 15, 2016 |
Title |
Saos2-p21WAF1/Cip1 Tet-ON_rep1 |
Sample type |
RNA |
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|
Source name |
Saos2-p21WAF1/Cip1 Tet-ON
|
Organism |
Homo sapiens |
Characteristics |
cell line: Saos2 cell type: human osteosarcoma genotype/variation: p21WAF1/Cip1
|
Treatment protocol |
For p21WAF1/Cip1 expression induction, Saos2 p21WAF1/Cip1 cells were treated with 2μg/ml Doxycycline (Applichem).
|
Growth protocol |
Saos2 Tet-ON p21WAF1/Cip1 (human osteosarcoma) carrying Tet-ON were maintained in DMEM (Invitrogen) with 10% FCS (Invitrogen), 2mM l-glutamine (Invitrogen), and 100 μg/ml penicillin and streptomycin (Invitrogen) at 37°C and 5% CO2.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
The sample labeling was performed based on the manufacturer’s standard protocols.
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|
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Hybridization protocol |
The microarray hybridization was performed based on the manufacturer’s standard protocols.
|
Scan protocol |
The arrays were scanned by the Affymetrix Scanner 3000(Affymetrix)
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Data processing |
The data were analyzed with analyzed using Partek Genomics Suite. RMA normalization was performed.
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Submission date |
Oct 08, 2014 |
Last update date |
May 15, 2016 |
Contact name |
Prokopios Alexandros Polyzos |
E-mail(s) |
app2006@med.cornell.edu
|
Organization name |
Weill Cornell Medicine
|
Street address |
413 E 69th St
|
City |
New York |
ZIP/Postal code |
10021 |
Country |
USA |
|
|
Platform ID |
GPL17586 |
Series (1) |
GSE62166 |
Protracted p53-independent stimulation of p21WAF1/Cip1 fuels genomic instability by deregulating the replication licensing machinery |
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