|
| Status |
Public on May 15, 2016 |
| Title |
Saos2-p21WAF1/Cip1 Tet-ON_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Saos2-p21WAF1/Cip1 Tet-ON
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Saos2
cell type: human osteosarcoma
genotype/variation: p21WAF1/Cip1
|
| Treatment protocol |
For p21WAF1/Cip1 expression induction, Saos2 p21WAF1/Cip1 cells were treated with 2μg/ml Doxycycline (Applichem).
|
| Growth protocol |
Saos2 Tet-ON p21WAF1/Cip1 (human osteosarcoma) carrying Tet-ON were maintained in DMEM (Invitrogen) with 10% FCS (Invitrogen), 2mM l-glutamine (Invitrogen), and 100 μg/ml penicillin and streptomycin (Invitrogen) at 37°C and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The sample labeling was performed based on the manufacturer’s standard protocols.
|
| |
|
| Hybridization protocol |
The microarray hybridization was performed based on the manufacturer’s standard protocols.
|
| Scan protocol |
The arrays were scanned by the Affymetrix Scanner 3000(Affymetrix)
|
| Data processing |
The data were analyzed with analyzed using Partek Genomics Suite. RMA normalization was performed.
|
| |
|
| Submission date |
Oct 08, 2014 |
| Last update date |
May 15, 2016 |
| Contact name |
Prokopios Alexandros Polyzos |
| E-mail(s) |
app2006@med.cornell.edu
|
| Organization name |
Weill Cornell Medicine
|
| Street address |
413 E 69th St
|
| City |
New York |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE62166 |
Protracted p53-independent stimulation of p21WAF1/Cip1 fuels genomic instability by deregulating the replication licensing machinery |
|
