|
| Status |
Public on Mar 10, 2007 |
| Title |
585pos3 tumor |
| Sample type |
RNA |
| |
|
| Source name |
mammary gland
|
| Organism |
Mus musculus |
| Characteristics |
mouse breast cancer
This animal synthesizes the SV40 T/t- antigens selectively in the mammary gland epithelial cells. Strain: NMRI, female, breast cancer.
|
| Treatment protocol |
breast cancer
|
| Growth protocol |
breast cancer
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAzol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The doublestranded DNA was transcribed in vitro into biotinylated cRNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430A 2.0 Array. Washing steps for the chip, staining with streptavidin-phycoerythrin and signal-amplification were performed according to the manufacturer’s instructions.
|
| Scan protocol |
Each hybridized Affymetrix GeneChip array was scanned with GeneChip Scanner 3000.
|
| Description |
see: Comparison of gene expression data from human and mouse breast cancers: Identification of a conserved breast tumor gene set. International Journal of Cancer
|
| Data processing |
The raw experimental microarray data were normalized with the Affymetrix GeneChip operating software based on the housekeeping gene method. Further analyses were performed with the software “CorrXpression”.
|
| |
|
| Submission date |
Dec 20, 2006 |
| Last update date |
Mar 18, 2009 |
| Contact name |
Andreas Klein |
| E-mail(s) |
andreas.klein@charite.de
|
| Phone |
+49 30 450 528087
|
| Fax |
+49 30 450 52945
|
| URL |
http://www.charite.de/molbiol/bioinf/tumbiol/
|
| Organization name |
Charité (CCO)
|
| Department |
Institute of Biochemistry
|
| Street address |
Charitéplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
|
| Platform ID |
GPL8321 |
| Series (2) |
| GSE6581 |
Expression data from mammary glands of transgenic mice |
| GSE6772 |
Comparison of gene expression data from human and mouse breast cancers |
|
