|
Status |
Public on Oct 16, 2014 |
Title |
patient 31 C14 |
Sample type |
RNA |
|
|
Source name |
Normal Skin Tissues
|
Organism |
Homo sapiens |
Characteristics |
population: Uyghyr gender: man age: 44 years tissue type: normal skin
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was harvested using TRIzol (Invitrogen) and miRNeasy mini kit (QIAGEN) according to manufacturer’s instructions.
|
Label |
Hy3
|
Label protocol |
After having passed RNA quantity measurement using the NanoDrop 1000, the samples were labeled using the miRCURY™ Hy3™/Hy5™ Power labeling kit and hybridized on the miRCURY™ LNA Array (v.18.0)
|
|
|
Hybridization protocol |
After stopping the labeling procedure, the Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (v.18.0) (Exiqon) according to array manual.
|
Scan protocol |
Following hybridization, the slides were achieved, washed several times using Wash buffer kit (Exiqon), and finally dried by centrifugation for 5 min at 400 rpm. Then the slides were scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).
|
Description |
After having passed RNA quantity measurement using the NanoDrop 1000, the samples were labeled using the miRCURY™ Hy3™/Hy5™ Power labeling kit and hybridized on the miRCURY™ LNA Array (v.18.0) which contains probes for 3100 mature miRNA.
|
Data processing |
Scanned images were then imported into GenePix Pro 6.0 software (Axon) for grid alignment and data extraction. Replicated miRNAs were averaged and miRNAs that intensities>=30 in all samples were chosen for calculating normalization factor. Expressed data were normalized using the Median normalization. After normalization, significant differentially expressed miRNAs were identified through Volcano Plot filtering. Hierarchical clustering was performed using MEV software (v4.6, TIGR). Only those with a greater than 2-fold increase or 2-fold decrease in expression in two paired samples were considered significantly changed.
|
|
|
Submission date |
Oct 15, 2014 |
Last update date |
Oct 16, 2014 |
Contact name |
Xiu-Juan Wu |
E-mail(s) |
xjwuxj@sina.com
|
Phone |
86-991-8563351
|
Organization name |
People’s Hospital of Xinjiang, Uygur Autonomous Region
|
Department |
Department of Dermatology
|
Street address |
91 Tianchi Road
|
City |
Urumqi |
State/province |
Xinjiang |
ZIP/Postal code |
830001 |
Country |
China |
|
|
Platform ID |
GPL19128 |
Series (1) |
GSE62354 |
MicroRNA Profiles and Differential Expression in Normal Skin Samples from the Uyghur and Han Populations in Xinjiang |
|