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Sample GSM1527310 Query DataSets for GSM1527310
Status Public on Mar 18, 2015
Title DOHH2-DMSO-1
Sample type RNA
 
Source name DOHH2 cell lines treated with DMSO
Organism Homo sapiens
Characteristics cell line: DOHH2
cell type: lymphoma cell line
treated with: DMSO
Treatment protocol treatment with pan-histone deacetylase inhibitor (HDACi), a thioacetate-ω (γ-lactam amide) derivative.
Growth protocol in vitro
Extracted molecule total RNA
Extraction protocol RNA extraction was performed using a commercially available kit (RNeasy, Qiagen, Frederick,MD) according to the manufactures’ recommendations. RNA quality was confirmed based on a RNA integrity number >8 by use of an electrophoresis bioanalyzer (2100 Agilent Bioanalyser).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion TotalPrep RNA Amplification Kit for Illumina arrays
 
Hybridization protocol Whole Genome Gene Expression Direct Hybridization
Scan protocol Illumina HiScan
Description 9287794016_A
Data processing Signal calculated by GenomeStudio Data Analysis Software’s Gene Expression Module (GSGX) Version 1.5.4 (Illumina, Inc.)
The data were normalised using quantile method
 
Submission date Oct 17, 2014
Last update date Mar 18, 2015
Contact name Francesco Bertoni
E-mail(s) francesco.bertoni@ior.usi.ch
Phone +41 91 820 0367
Organization name IOR
Department Lymphoma & Genomics Research Program
Street address Via V. Vela 6
City Bellinzona
State/province Ticino
ZIP/Postal code 6500
Country Switzerland
 
Platform ID GPL10558
Series (1)
GSE62460 Preclinical antitumor activity of ST7612AA1: a novel second generation oral histone deacetylase (HDAC) inhibitor

Data table header descriptions
ID_REF
VALUE Log2 Normalized Data

Data table
ID_REF VALUE
ILMN_1762337 6.597849189
ILMN_2055271 6.727767959
ILMN_1736007 6.576368035
ILMN_2383229 6.490239063
ILMN_1806310 6.61414043
ILMN_1779670 6.582544481
ILMN_1653355 6.532959289
ILMN_1717783 6.502098383
ILMN_1705025 6.597204905
ILMN_1814316 6.721006546
ILMN_2359168 6.572012665
ILMN_1731507 6.595784322
ILMN_1787689 6.566920135
ILMN_3241953 7.416041112
ILMN_1745607 6.531282473
ILMN_2136495 6.496684445
ILMN_1668111 6.559265125
ILMN_2295559 6.553334189
ILMN_1735045 6.555313173
ILMN_1680754 6.644113666

Total number of rows: 47231

Table truncated, full table size 1148 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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