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Sample GSM1527311 Query DataSets for GSM1527311
Status Public on Mar 18, 2015
Title DOHH2-DMSO-2
Sample type RNA
 
Source name DOHH2 cell lines treated with DMSO
Organism Homo sapiens
Characteristics cell line: DOHH2
cell type: lymphoma cell line
treated with: DMSO
Treatment protocol treatment with pan-histone deacetylase inhibitor (HDACi), a thioacetate-ω (γ-lactam amide) derivative.
Growth protocol in vitro
Extracted molecule total RNA
Extraction protocol RNA extraction was performed using a commercially available kit (RNeasy, Qiagen, Frederick,MD) according to the manufactures’ recommendations. RNA quality was confirmed based on a RNA integrity number >8 by use of an electrophoresis bioanalyzer (2100 Agilent Bioanalyser).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion TotalPrep RNA Amplification Kit for Illumina arrays
 
Hybridization protocol Whole Genome Gene Expression Direct Hybridization
Scan protocol Illumina HiScan
Description 9287794016_C
Data processing Signal calculated by GenomeStudio Data Analysis Software’s Gene Expression Module (GSGX) Version 1.5.4 (Illumina, Inc.)
The data were normalised using quantile method
 
Submission date Oct 17, 2014
Last update date Mar 18, 2015
Contact name Francesco Bertoni
E-mail(s) francesco.bertoni@ior.usi.ch
Phone +41 91 820 0367
Organization name IOR
Department Lymphoma & Genomics Research Program
Street address Via V. Vela 6
City Bellinzona
State/province Ticino
ZIP/Postal code 6500
Country Switzerland
 
Platform ID GPL10558
Series (1)
GSE62460 Preclinical antitumor activity of ST7612AA1: a novel second generation oral histone deacetylase (HDAC) inhibitor

Data table header descriptions
ID_REF
VALUE Log2 Normalized Data

Data table
ID_REF VALUE
ILMN_1762337 6.621774671
ILMN_2055271 6.703203612
ILMN_1736007 6.613017132
ILMN_2383229 6.59080077
ILMN_1806310 6.513165253
ILMN_1779670 6.502656536
ILMN_1653355 6.555653273
ILMN_1717783 6.511311359
ILMN_1705025 6.63598707
ILMN_1814316 6.551716195
ILMN_2359168 6.506171916
ILMN_1731507 6.537556578
ILMN_1787689 6.6526623
ILMN_3241953 7.225724096
ILMN_1745607 6.65839041
ILMN_2136495 6.485095653
ILMN_1668111 6.519422746
ILMN_2295559 6.653775201
ILMN_1735045 6.557470249
ILMN_1680754 6.713035069

Total number of rows: 47231

Table truncated, full table size 1147 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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