|
| Status |
Public on Mar 18, 2015 |
| Title |
DOHH2-DMSO-2 |
| Sample type |
RNA |
| |
|
| Source name |
DOHH2 cell lines treated with DMSO
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: DOHH2
cell type: lymphoma cell line
treated with: DMSO
|
| Treatment protocol |
treatment with pan-histone deacetylase inhibitor (HDACi), a thioacetate-ω (γ-lactam amide) derivative.
|
| Growth protocol |
in vitro
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed using a commercially available kit (RNeasy, Qiagen, Frederick,MD) according to the manufactures’ recommendations. RNA quality was confirmed based on a RNA integrity number >8 by use of an electrophoresis bioanalyzer (2100 Agilent Bioanalyser).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep RNA Amplification Kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Whole Genome Gene Expression Direct Hybridization
|
| Scan protocol |
Illumina HiScan
|
| Description |
9287794016_C
|
| Data processing |
Signal calculated by GenomeStudio Data Analysis Software’s Gene Expression Module (GSGX) Version 1.5.4 (Illumina, Inc.)
The data were normalised using quantile method
|
| |
|
| Submission date |
Oct 17, 2014 |
| Last update date |
Mar 18, 2015 |
| Contact name |
Francesco Bertoni |
| E-mail(s) |
francesco.bertoni@ior.usi.ch
|
| Phone |
+41 91 820 0367
|
| Organization name |
IOR
|
| Department |
Lymphoma & Genomics Research Program
|
| Street address |
Via V. Vela 6
|
| City |
Bellinzona |
| State/province |
Ticino |
| ZIP/Postal code |
6500 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE62460 |
Preclinical antitumor activity of ST7612AA1: a novel second generation oral histone deacetylase (HDAC) inhibitor |
|
