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Sample GSM1527312 Query DataSets for GSM1527312
Status Public on Mar 18, 2015
Title DOHH2-DMSO-3
Sample type RNA
 
Source name DOHH2 cell lines treated with DMSO
Organism Homo sapiens
Characteristics cell line: DOHH2
cell type: lymphoma cell line
treated with: DMSO
Treatment protocol treatment with pan-histone deacetylase inhibitor (HDACi), a thioacetate-ω (γ-lactam amide) derivative.
Growth protocol in vitro
Extracted molecule total RNA
Extraction protocol RNA extraction was performed using a commercially available kit (RNeasy, Qiagen, Frederick,MD) according to the manufactures’ recommendations. RNA quality was confirmed based on a RNA integrity number >8 by use of an electrophoresis bioanalyzer (2100 Agilent Bioanalyser).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion TotalPrep RNA Amplification Kit for Illumina arrays
 
Hybridization protocol Whole Genome Gene Expression Direct Hybridization
Scan protocol Illumina HiScan
Description 9287794016_E
Data processing Signal calculated by GenomeStudio Data Analysis Software’s Gene Expression Module (GSGX) Version 1.5.4 (Illumina, Inc.)
The data were normalised using quantile method
 
Submission date Oct 17, 2014
Last update date Mar 18, 2015
Contact name Francesco Bertoni
E-mail(s) francesco.bertoni@ior.usi.ch
Phone +41 91 820 0367
Organization name IOR
Department Lymphoma & Genomics Research Program
Street address Via V. Vela 6
City Bellinzona
State/province Ticino
ZIP/Postal code 6500
Country Switzerland
 
Platform ID GPL10558
Series (1)
GSE62460 Preclinical antitumor activity of ST7612AA1: a novel second generation oral histone deacetylase (HDAC) inhibitor

Data table header descriptions
ID_REF
VALUE Log2 Normalized Data

Data table
ID_REF VALUE
ILMN_1762337 6.615039293
ILMN_2055271 6.719503736
ILMN_1736007 6.649314714
ILMN_2383229 6.561486804
ILMN_1806310 6.551878095
ILMN_1779670 6.588530679
ILMN_1653355 6.537958739
ILMN_1717783 6.500084778
ILMN_1705025 6.553251747
ILMN_1814316 6.600432868
ILMN_2359168 6.490061095
ILMN_1731507 6.502156395
ILMN_1787689 6.607431361
ILMN_3241953 7.220030272
ILMN_1745607 6.523993088
ILMN_2136495 6.589494153
ILMN_1668111 6.499777664
ILMN_2295559 6.631713561
ILMN_1735045 6.610710979
ILMN_1680754 6.659461585

Total number of rows: 47231

Table truncated, full table size 1147 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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