|
| Status |
Public on Oct 21, 2014 |
| Title |
mouse lung infected 5 days, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse lung infected 5 days with PR8 H1N1 influenza virus
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lung
strain: ICR
|
| Treatment protocol |
Whole mouse lung tissues were homogenized in liquid nitrogen. Total RNAs were extracted from mouse lungs using QIAGEN RNeasy Mini Kit.
|
| Growth protocol |
Mice were anesthetized with ether and were intranasally inoculated with 104 TCID50 of PR8 virus in physiological saline.For the total RNAs extraction, entire lungs from mice infected by PR8 virus were randomly selected and harvested on the 2th and 5th day.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted from mouse lungs using QIAGEN RNeasy Mini Kit,according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip miRNA 4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
|
| Description |
13～15g
microRNA expression data
|
| Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Oct 20, 2014 |
| Last update date |
Oct 21, 2014 |
| Contact name |
yanyan bao |
| E-mail(s) |
baoyanyan885@126.com
|
| Organization name |
Biosafety Laboratory, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences
|
| Street address |
Dongzhimen South Street
|
| City |
beijing |
| ZIP/Postal code |
100700 |
| Country |
China |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE62495 |
MicroRNA expression data from H1N1 influenza virus infected mouse lung |
|
