|
| Status |
Public on Sep 29, 2017 |
| Title |
Sample 7 - hlk - Z20 |
| Sample type |
RNA |
| |
|
| Source name |
FACS purified GFP+ T-lymphoblasts
|
| Organism |
Danio rerio |
| Characteristics |
model: hlk sample
cell type: Sorted GFP+ T-lymphoblasts
|
| Treatment protocol |
FACS purified GFP+ T-lymphoblasts from the bodies of hMYC-ER and hlk zebrafish models at the final stage of disease
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction using Trizol protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12,5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Zebrafish Genome Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450DX.
|
| Scan protocol |
GeneChips were scanned using the Scan GCS3000.
|
| Description |
Gene expression data from FACS purified GFP+ T-lymphoblasts of hlk specimen at the final stage of disease
|
| Data processing |
The data were normalized using R software [www.r-ptoject.org] with BioConductor package [www.bioconductor.org]
|
| |
|
| Submission date |
Oct 21, 2014 |
| Last update date |
Sep 29, 2017 |
| Contact name |
Chiara Borga |
| E-mail(s) |
chiaraborga@gmail.com
|
| Organization name |
University of Padua
|
| Street address |
Corso Stati Uniti,4
|
| City |
Padova |
| ZIP/Postal code |
35127 |
| Country |
Italy |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE62541 |
Discovery of Central Nervous System Infiltration in Zebrafish T Cell Acute Lymphoblastic Leukemia |
|
